Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Comparison of Human-Induced Pluripotent Stem Cells and Mesenchymal Stem Cell Differentiation Potential to Insulin Producing Cells in 2D and 3D Culture Systems in Vitro Publisher Pubmed



Abazari MF1 ; Nasiri N2 ; Nejati F2 ; Zare Karizi S3 ; Amini Faskhodi M4 ; Saburi E5 ; Aghapur N6 ; Mahdavi MR7 ; Ardeshirylajimi A8 ; Enderami SE9, 10 ; Soleimanifar F11, 12
Authors
Show Affiliations
Authors Affiliations
  1. 1. Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
  3. 3. Department of biology, Varamin Pishva branch, Islamic Azad University, Pishva, Varamin, Iran
  4. 4. Department of Biology, Tehran Medical Branch, Islamic Azad University, Tehran, Iran
  5. 5. Medical Genetics and Molecular Medicine Department, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  6. 6. Department of Biotechnology, Faculty of Science, University of Tehran, Tehran, Iran
  7. 7. Thalassemia Research Center, Mazandaran University of Medical Sciences, Sari, Iran
  8. 8. Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  9. 9. Diabetes Research Center, Mazandaran University of Medical Sciences, Sari, Iran
  10. 10. Immunogenetics Research Center, Department of Medical Biotechnology, Mazandaran University of Medical Sciences, Sari, Iran
  11. 11. Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran
  12. 12. Department of Medical Biotechnology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran

Source: Journal of Cellular Physiology Published:2020


Abstract

Diabetes is one of the most common diseases in the world that is chronic, progressive, and costly, and causes many complications. Common drug therapies are not able to cure it, and pancreas transplantation is not responsive to the high number of patients. The production of the insulin producing cells (IPCs) from the stem cells in the laboratory and their transplantation to the patient's body is one of the most promising new approaches. In this study, the differentiation potential of the induced pluripotent stem cells (iPSCs) and mesenchymal stem cells (MSCs) into IPCs was compared to each other while cultured on poly(lactic-co-glycolic) acid (PLGA)/polyethylene glycol (PEG) nanofibrous scaffold as a 3D substrate and tissue culture polystyrene (TCPS) as a 2D substrate. Although the expression level of the insulin, Glut2 and pdx-1 genes in stem cells cultured on 3D substrate was significantly higher than the stem cells cultured on 2D substrate, the highest expression level of these genes was detected in the iPSCs cultured on PLGA-PEG. Insulin and C-peptide secretions from differentiated cells were also investigated and the results showed that secretions in cultured iPSCs on the PLGA-PEG were significantly higher than cultured iPSCs on the TCPS and cultured MSCs on both PLGA-PEG and TCPS. In addition, insulin protein was also expressed in the cultured iPSCs on the PLGA-PEG significantly higher than cultured MSCs on the PLGA-PEG. It can be concluded that differentiation potential of iPSCs into IPCs is significantly higher than human MSCs at both 2D and 3D culture systems. © 2019 Wiley Periodicals, Inc.
Related Docs
View other Related Docs
1. Generation of High Yield Insulin-Producing Cells (Ipcs) From Various Sources of Stem Cells, Vitamins and Hormones (2021)
2. Pcl/Pva Nanofibrous Scaffold Improve Insulin-Producing Cells Generation From Human Induced Pluripotent Stem Cells, Gene (2018)
3. Collagen Coated Electrospun Polyethersulfon Nanofibers Improved Insulin Producing Cells Differentiation Potential of Human Induced Pluripotent Stem Cells, Artificial Cells# Nanomedicine and Biotechnology (2018)
4. Three-Dimensional Nanofiberous Plla/Pcl Scaffold Improved Biochemical and Molecular Markers Hips Cell-Derived Insulin-Producing Islet-Like Cells, Artificial Cells# Nanomedicine and Biotechnology (2018)
5. The Role of Micrornas in the Induction of Pancreatic Differentiation, Current Stem Cell Research and Therapy (2020)
6. Platelet-Rich Plasma Incorporated Electrospun Pva-Chitosan-Ha Nanofibers Accelerates Osteogenic Differentiation and Bone Reconstruction, Gene (2019)
7. Generation of Insulin-Producing Cells From Human Induced Pluripotent Stem Cells on Plla/Pva Nanofiber Scaffold, Artificial Cells# Nanomedicine and Biotechnology (2018)
8. Nanofiber-Based Systems Intended for Diabetes, Journal of Nanobiotechnology (2021)
Experts (# of related papers)
View all Related Experts
Mahboubeh Parsaeian (1)
Ghodratollah Panahi (1)
Other Related Docs
9. Improved Osteogenic Differentiation of Human Induced Pluripotent Stem Cells Cultured on Polyvinylidene Fluoride/Collagen/Platelet-Rich Plasma Composite Nanofibers, Journal of Cellular Physiology (2020)
10. Generation of High-Yield Insulin Producing Cells From Human-Induced Pluripotent Stem Cells on Polyethersulfone Nanofibrous Scaffold, Artificial Cells# Nanomedicine and Biotechnology (2018)
11. A Novel Silk/Pes Hybrid Nanofibrous Scaffold Promotes the in Vitro Proliferation and Differentiation of Adipose-Derived Mesenchymal Stem Cells Into Insulin Producing Cells, Polymers for Advanced Technologies (2020)
12. Micro-Rna-Incorporated Electrospun Nanofibers Improve Osteogenic Differentiation of Human-Induced Pluripotent Stem Cells, Journal of Biomedical Materials Research - Part A (2020)
13. Bladder Smooth Muscle Cell Differentiation of the Human Induced Pluripotent Stem Cells on Electrospun Poly(Lactide-Co-Glycolide) Nanofibrous Structure, Gene (2019)
14. Phbv Nanofibers Promotes Insulin-Producing Cells Differentiation of Human Induced Pluripotent Stem Cells, Gene (2021)
15. Improvement of Hepatogenic Differentiation of Ips Cells on an Aligned Polyethersulfone Compared to Random Nanofibers, Artificial Cells# Nanomedicine and Biotechnology (2018)
16. Induced Pluripotent Stem Cells As a New Getaway for Bone Tissue Engineering: A Systematic Review, Cell Proliferation (2017)
17. Efficient Osteogenic Differentiation of the Dental Pulp Stem Cells on Β-Glycerophosphate Loaded Polycaprolactone/Polyethylene Oxide Blend Nanofibers, Journal of Cellular Physiology (2019)
18. Induced Pluripotent Stem Cell-Derived Extracellular Vesicles: A Novel Approach for Cell-Free Regenerative Medicine, Journal of Cellular Physiology (2019)
19. Advancing Mesenchymal Stem Cell Therapy With Crispr/Cas9 for Clinical Trial Studies, Advances in Experimental Medicine and Biology (2020)
20. In Vitro Osteogenic Differentiation Potential of the Human Induced Pluripotent Stem Cells Augments When Grown on Graphene Oxide-Modified Nanofibers, Gene (2019)
21. Improved Bladder Smooth Muscle Cell Differentiation of the Mesenchymal Stem Cells When Grown on Electrospun Polyacrylonitrile/Polyethylene Oxide Nanofibrous Scaffold, Journal of Cellular Biochemistry (2019)
22. Trimetazidine Preconditioning Potentiates the Effect of Mesenchymal Stem Cells Secretome on the Preservation of Rat Pancreatic Islet Survival and Function in Vitro, Applied Biochemistry and Biotechnology (2023)
23. Microrna-2861 and Nanofibrous Scaffold Synergistically Promote Human Induced Pluripotent Stem Cells Osteogenic Differentiation, Polymers for Advanced Technologies (2020)
24. Decellularized Amniotic Membrane Scaffolds Improve Differentiation of Ipscs to Functional Hepatocyte-Like Cells, Journal of Cellular Biochemistry (2020)
25. The Clinical Trials of Mesenchymal Stromal Cells Therapy, Stem Cells International (2021)
26. Therapeutic Approaches of Cell Therapy Based on Stem Cells and Terminally Differentiated Cells: Potential and Effectiveness, Cells and Development (2024)
27. Improved Stem Cell Therapy of Spinal Cord Injury Using Gdnf-Overexpressed Bone Marrow Stem Cells in a Rat Model, Biologicals (2017)
28. Efficient Cardiomyocyte Differentiation of Induced Pluripotent Stem Cells on Plga Nanofibers Enriched by Platelet-Rich Plasma, Polymers for Advanced Technologies (2021)
29. Worldwide Trends in Diabetes Since 1980: A Pooled Analysis of 751 Population-Based Studies With 4.4 Million Participants, The Lancet (2016)
30. Recent Advances in Mesenchymal Stem Cell Therapy for Multiple Sclerosis: Clinical Applications and Challenges, Frontiers in Cell and Developmental Biology (2025)
31. Metal-Based Nanostructures/Plga Nanocomposites: Antimicrobial Activity, Cytotoxicity, and Their Biomedical Applications, ACS Applied Materials and Interfaces (2020)
32. The Generation of Islet-Like Insulin-Producing Cells From Wharton's Jelly-Derived Mesenchymal Stem Cells on the Pes/Fish Gelatin Scaffold, Regenerative Therapy (2024)
33. Toward in Vitro Production of Platelet From Induced Pluripotent Stem Cells, Stem Cell Reviews and Reports (2022)
34. Hybrid Poly-L-Lactic Acid/Poly(Ε-Caprolactone) Nanofibrous Scaffold Can Improve Biochemical and Molecular Markers of Human Induced Pluripotent Stem Cell-Derived Hepatocyte-Like Cells, Journal of Cellular Physiology (2019)
35. Poly (3-Hydroxybutyrate-Co-3-Hydroxyvalerate) Improved Osteogenic Differentiation of the Human Induced Pluripotent Stem Cells While Considered As an Artificial Extracellular Matrix, Journal of Cellular Physiology (2019)
36. Signaling Crosstalk Between Mesenchymal Stem Cells and Tumor Cells: Implications for Tumor Suppression or Progression, Cytokine and Growth Factor Reviews (2024)
37. Bone Tissue Engineering: Adult Stem Cells in Combination With Electrospun Nanofibrous Scaffolds, Journal of Cellular Physiology (2018)
38. Immunomodulatory Activity of Human Bone Marrow and Adipose-Derived Mesenchymal Stem Cells Prolongs Allogenic Skin Graft Survival in Nonhuman Primates, Cell Journal (2021)
39. Stem Cell Therapy Potency in Personalizing Severe Covid-19 Treatment, Stem Cell Reviews and Reports (2021)
40. Biological Behavior of the Curcumin Incorporated Chitosan/Poly(Vinyl Alcohol) Nanofibers for Biomedical Applications, Journal of Cellular Biochemistry (2019)
41. Applied Induced Pluripotent Stem Cells in Combination With Biomaterials in Bone Tissue Engineering, Journal of Cellular Biochemistry (2017)
42. Molecular Study of the Proliferation Process of Beta Cells Derived From Pluripotent Stem Cells, Molecular Biology Reports (2022)