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Improved Osteogenic Differentiation of Human Induced Pluripotent Stem Cells Cultured on Polyvinylidene Fluoride/Collagen/Platelet-Rich Plasma Composite Nanofibers Publisher Pubmed



Abazari MF1 ; Soleimanifar F2 ; Amini Faskhodi M3 ; Mansour RN4 ; Amini Mahabadi J5 ; Sadeghi S6 ; Hassannia H7, 8 ; Saburi E9 ; Enderami SE10 ; Khani MM11 ; Zare Karizi S12
Authors
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Authors Affiliations
  1. 1. Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran
  3. 3. Department of Biology, Tehran Medical Branch, Islamic Azad University, Tehran, Iran
  4. 4. Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
  5. 5. Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran
  6. 6. Recombinant Proteins Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
  7. 7. Immunogenetics Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  8. 8. Amol Faculty of Paramedical Sciences, Mazandaran University of Medical Sciences, Sari, Iran
  9. 9. Department of Medical Genetics and Molecular Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  10. 10. Immunogenetics Research Center, Department of Medical Biotechnology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  11. 11. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  12. 12. Department of Biology, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran

Source: Journal of Cellular Physiology Published:2020


Abstract

Blood transfusion or blood products, such as plasma, have a long history in improving health, but today, platelet-rich plasma (PRP) is used in various medical areas such as surgery, orthopedics, and rheumatology in many ways. Considering the high efficiency of tissue engineering in repairing bone defects, in this study, we investigated the combined effect of nanofibrous scaffolds in combination with PRP on the osteogenic differentiation potential of human induced pluripotent stem cells (iPSCs). Electrospinning was used for fabricating nanofibrous scaffolds by polyvinylidene fluoride/collagen (PVDF/col) with and without PRP. After scaffold characterization, the osteoinductivity of the fabricated scaffolds was studied by culturing human iPSCs under osteogenic medium. The results showed that PRP has a considerable positive effect on the biocompatibility of the PVDF/col nanofibrous scaffold when examined by protein adsorption, cell attachment, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. In addition, the results obtained from alkaline phosphatase activity and calcium content assays demonstrated that nanofibers have higher osteoinductivity while grown on PRP-incorporated PVDF/col nanofibers. These results were also confirmed while the osteogenic differentiation of the iPSCs was more investigated by evaluating the most important bone-related genes expression level. According to the results, it can be concluded that PVDF/col/PRP has much more osteoinductivity while compared with the PVDF/col, and it can be introduced as a promising bone bio-implant for use in bone tissue engineering applications. © 2019 Wiley Periodicals, Inc.
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