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Isolation and Evaluation of Dental Pulp Stem Cells From Teeth With Advanced Periodontal Disease Pubmed



Derakhshani A1 ; Raoof M2 ; Dabiri S1 ; Farsinejad AR1 ; Gorjestani H3 ; Yaghoobi MM4 ; Shokouhinejad N5 ; Ehsani M6
Authors
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Authors Affiliations
  1. 1. Pathology and Stem Cell Research center, Kerman University of Medical Sciences, Kerman, Iran
  2. 2. Laboratory of Molecular Neuroscience, Neuroscience Research Center, Institute of Neuropharmacology/Department of Endodontics, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran
  3. 3. Private Practice. Former Assistant Professor of Department of Endodontics, School of Dentistry, Kerman University of Medical Sciences, Kerman, Iran
  4. 4. Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
  5. 5. Dental Research Center, Dentistry Research Institute, Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Dental Material Research Center, Babol, Iran

Source: Archives of Iranian Medicine Published:2015


Abstract

Introduction:Successful isolation of mesenchymal stem cells from waste tissues might be extremely promising for developing stem cell-based therapies. This study aimed to explore whether cells retrieved from teeth extracted due to advanced periodontal disease present mesenchymal stem cell-like properties. Methods: Pulp cells were isolated from 15 intact molars and 15 teeth with advanced periodontal disease. Cell proliferation and mark­ers of mesenchymal stem cells were evaluated. Results: Based on the RT-PCR and agarose gel electrophoresis, nucleostemin, Oct-4 and jmj2c, but not Nanog, were expressed in undifferentiated mesenchymal stem cells of both groups. Interestingly, diseased pulp exhibited higher gene expressions although it was not statistically significant. The average percentage of BrdU positive cells in the diseased group (84.4%, n = 5) was significantly higher than that of the control group (65.4%, n = 5) (f-test, P = 0.001). Conclusion: Our results indicate the successful isolation of mesenchymal stem cells from the pulp tissue of hopeless periodontally involved teeth. © 2015 Academy of Medical Sciences of I.R. Iran. All rights reserved.
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