Role of Rosemary Officinalis in the Hepatotoxicity Induced by Chlorpyrifos Sub-Chronic Exposure in Rats



Pourbabaki R¹ ; Khadem M¹ ; Samiei S¹ ; Amirkhanloo F² ; Shahtaheri SJ³ Show Affiliations
Source: Iran Occupational Health Published:2021

Abstract

Background and aims: Chlorpyrifos is a worldwide used organophosphorus pesticide. In addition to controlling the cholinesterase enzyme, this toxicant also plays a significant role in the oxidative stress and augmented oxidants. Whereas, extracted rosemary is an antioxidant, and plays a protective role against free radicals. Therefore, the object of this study was to investigate the reduction of liver damage caused by chlorpyrifos through the effect of the rosemary extract on the oxidative stress of this pesticide. Methods: In this study, 30 male wistar rats, weighing 200-220 g were used. The animals (Rats)were randomly divided into five groups, each group containing six rats for further comparison; Group І: served as control, Group П: the chlorpyrifos exposure group (13.5 mg / kg single dose), Group Ш: the group exposed to the rosemary extract (100 mg / kg), Group ІV: the exposure group with the toxicant (13.5 mg / kg dose) and rosemary (100 mg / kg concentration), Group V: simultaneous exposure of toxicant and rosemary (200 mg / kg oral dose). After 30 days, the Rats were weighed, killed and the liver tissue was histologically examined and the changes in MDA and GPx were reviewed. Results: The results of statistical tests showed that, compared to the control group, the chlorpyrifos significantly damaged the liver tissue and altered the activity of the glutathione peroxidase and malondialdehyde. The simultaneous exposure to CPF and rosemary extract can significantly make modifications to the GPx and MDA levels and mitigate the CPF damage. Conclusion: According to the results, the rosemary extract can reduce the oxidative stress caused by the chlorpyrifos and this plant can be used to treat poisoning caused by this toxic pesticide. Copyright © 2021 The Authors.