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The Effect of Purmorphamine on Differentiation of Endometrial Stem Cells Into Osteoblast-Like Cells on Collagen/Hydroxyapatite Scaffolds Publisher Pubmed



Bahrami N1, 2, 3 ; Malekolkottab F4 ; Ebrahimibarough S5 ; Alizadeh Tabari Z4 ; Hamisi J4 ; Kamyab A6 ; Mohamadnia A7, 8 ; Ai A1 ; Bayat F1 ; Bahrami N1, 2, 3 ; Ai J5
Authors
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Authors Affiliations
  1. 1. Department of Oral and Maxillofacial Surgery, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Craniomaxillo Facial Research Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Iranian Tissue Bank and Research Center, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Periodontics, School of Dentistry, Qazvin University of Medical Sciences, Qazvin, Iran
  5. 5. Department of Tissue Engineering and Applied Cell Sciences, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Genetics, Science and Research Branch, Islamic Azad University, Tehran, Iran
  7. 7. Virology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
  8. 8. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  9. 9. Department of Biomedical Engineering, Wake Forest university? Virginia Tech, Winston Salem, NC, United States

Source: Artificial Cells# Nanomedicine and Biotechnology Published:2017


Abstract

We assessed the effect of purmorphamine along with collagen/hydroxyapatite scaffold in inducing osteogenesis of human endometrial stem cells (hEnSCs). The adhesion, viability, proliferation, and differentiation of cells on scaffold were assayed with SEM, MTT, real time-PCR, and ALP assay, respectively. The results were shown good integration of cells with scaffold. Also, qRT-PCR of differentiated cells shows that osteoblast cell markers are expressed after 21d in 2D and scaffold groups while in the scaffold group the expression of these markers were higher than the 2D group. Based on our findings, collagen/hydroxyapatite scaffold with PMA has the potential role in osteogenic differentiation of hEnSCs. © 2016 Informa UK Limited, trading as Taylor & Francis Group.
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