Antibacterial Amorphous Magnesium Phosphate/Graphene Oxide for Accelerating Bone Regeneration Publisher

Summary: A study found graphene-enhanced magnesium phosphate slows degradation, boosting bone repair. #BoneHealth #Biomaterials

Pahlevanzadeh F¹ ; Emadi R¹ ; Setayeshmehr M² ; Kharaziha M¹ ; Poursamar SA²
Source: Biomaterials Advances Published:2022

Abstract

Magnesium phosphates (Mg[sbnd]P)s have attracted interest as an alternative biomaterial compared to the calcium phosphate (Ca[sbnd]P)s compounds in the bone regeneration application in terms of their prominent biodegradability, lack of cytotoxicity, and ability of bone repair stimulation. Among them, amorphous magnesium phosphates (AMP)s indicated a higher rate of resorption, while preserving high osteoblasts viability and proliferation, which is comparable to their Ca[sbnd]P peers. However, fast degradation of AMP leads to the initial fast release of Mg2+ ions and adverse effects on its excellent biological features. It seems that the addition of graphene oxide (GO) to magnesium phosphate can moderate its degradation rate. Hence, a novel in situ synthesized AMP powders containing 0.05, 0.25, 0.5, and 1 wt% of graphene oxide (AMP/GO) were developed to achieve a favorable degradation rate, desirable antibacterial properties against both Escherichia coli (E. coli), Staphylococcus aureus (S. aureus) accompanying with proper cell viability and proliferation. The incorporation of 0.5 wt% of graphene oxide into the AMP ceramic led to reduce the release of Mg2+ ions from 571.2 ± 12.9 mg/L to 372.8 ± 14.7 mg/L and P ions from 354.8 ± 11.9 mg/L to 245.3 ± 9.9 mg/L, at day 10 of immersion in PBS. Besides, AMP/0.5 GO bioceramics were capable of eradicating all bacterial colonies of both strains. On the other hand, MG63 cells viability went up from 143.46% ± 7.54 to 184.46% ± 11.54 on the 7th day of culture in the presence of 0.5 wt% of GO compared to pure AMP ceramic. Furthermore, alizarin red staining and alkaline phosphatase (ALP) activity demonstrated the ability of AMP/GO to maintain the osteogenic phenotype of MG63 cells during 7 days culture. Therefore, it can be concluded that well distributed and in situ synthesized AMP/0.5GO powders can be a promising biomaterial for bone tissue regeneration. © 2022 Elsevier B.V.