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The Methylation Status of Gata3 Potentially Predicts the Outcomes of Assisted Reproductive Technologies Publisher Pubmed



Beiraghdar M1 ; Beiraghdar M1 ; Khosravi S3, 4
Authors
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Authors Affiliations
  1. 1. Department of Biology, Faculty of Basic Science, Islamic Azad University of Center Tehran Branch, Tehran, Iran
  2. 2. Department of pathology, specialist of anatomical and clinical pathology, University of Isfahan, Isfahan, Iran
  3. 3. Department of Genetics and Molecular Biology, Isfahan University of Medical Science, Isfahan, Iran
  4. 4. Genetic Lab in Majesty of Maryam Infertility Center, Martyr Beheshti Hospital, Isfahan University of Medical Science, Isfahan, Iran

Source: Human Fertility Published:2023


Abstract

Evaluation of methylation status of genes in sperm samples has been suggested for diagnosis of male infertility as well as prognosis of assisted reproductive technologies (ART) outcomes. In this study, we compared the methylation pattern of the GATA3 gene in infertile and fertile men as well as in infertile men with positive and negative ART outcome based on clinical pregnancy. Ejaculates were obtained from 42 infertile men with a negative ART outcome (group 1), 30 infertile men with a positive ART outcome (group 2), and 21 fertile men (control). Then, samples were subjected to genomic DNA isolation and subsequent TUNEL assay and methylation-specific PCR. The number of infertile men with at least one methylated allele of GATA3 was significantly higher compared to the control group (p = 0.022). Also, the number of patients with at least one methylated allele was significantly higher in group 1 compared to group 2 (p = 0.013). Moreover, the TUNEL assay revealed that the amount of sperm DNA fragmentation is higher in group 1 compared to group 2 (p = 0.008). The findings of our study demonstrated that the degree of GATA3 methylation can potentially differentiate between infertile and fertile men and more importantly can potentially predict the outcome of ART. © 2023 The British Fertility Society.
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