Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Oncogene Musashi1 Encodes Novel Mirnas in Breast Cancer Publisher Pubmed



Lachinani L1 ; Forouzanfar M1 ; Dormiani K1 ; Soltani BM2 ; Dolatshahi K3 ; Hakimian SM4, 5 ; Dokanehiifard S6 ; Nasresfahani MH1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
  2. 2. Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
  3. 3. Department of Medicine, Najafabad Branch, Islamic Azad University, Najafabad, Iran
  4. 4. Ordibehesht Breast Clinic, Isfahan, Iran
  5. 5. Poursina Hakim Digestive Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  6. 6. Department of Human Genetics, Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, Biomedical Research Building, Miami, FL, United States

Source: Scientific Reports Published:2023


Abstract

RNA-binding protein Musashi1 (MSI1) shows an increased expression level in several cancers and has been introduced as a prognostic marker in some malignancies. It is expected that if any miRNA is encoded by this gene, it might have a role in cancer development or could be considered as a prognostic biomarker. Accordingly, in this study, we aimed to find novel miRNA(s) inside the intronic regions of the MSI1 gene. Here, we report two novel miRNAs within intron 4 of MSI1 gene, named MSM2 and MSM3, which were selected among several miRNA precursors predicted by bioinformatic studies. For experimental analysis, corresponding precursor miRNAs were transfected into HEK293T cells and exogenous expression of the mature miRNAs were detected. Two mature miRNAs, MSM3-3p and MSM3-5p were generated by MSM3 precursor and one, MSM2-5p was derived from MSM2. Besides, endogenous expression of MSM2-5p and MSM3-3p was detected in MCF-7 and SH-SY5Y cell lines. Expression of both mature miRNAs was also detected in clinical samples of breast cancer. Additionally, the interaction between the MSM3-3p and 3′UTR region of PDE11A was confirmed by dual luciferase assay. Overall, our data demonstrated that MSI1 gene encodes two novel miRNAs in breast cancer cells. © 2023, Springer Nature Limited.
Related Docs
View other Related Docs
1. Msi2 Expression Is Decreased in Grade Ii of Gastric Carcinoma, Pathology Research and Practice (2013)
2. Recent Insights Into the Microrna-Dependent Modulation of Gliomas From Pathogenesis to Diagnosis and Treatment, Cellular and Molecular Biology Letters (2022)
3. Cross-Talk Between Oxidative Stress Signaling and Microrna Regulatory Systems in Carcinogenesis: Focused on Gastrointestinal Cancers, Biomedicine and Pharmacotherapy (2020)
4. The Rna Binding Protein Musashi1 Regulates Apoptosis, Gene Expression and Stress Granule Formation in Urothelial Carcinoma Cells, Journal of Cellular and Molecular Medicine (2011)
5. The Stem Cell Self-Renewal Gene, Musashi 1, Is Highly Expressed in Tumor and Non-Tumor Samples of Human Bladder, Indian Journal of Cancer (2013)
6. The Importance of Snps at Mirna Binding Sites As Biomarkers of Gastric and Colorectal Cancers: A Systematic Review, Journal of Personalized Medicine (2022)
7. Dysregulated Expression and Functions of Microrna-330 in Cancers: A Potential Therapeutic Target, Biomedicine and Pharmacotherapy (2022)
8. Microrna Dysregulation and Its Impact on Apoptosis-Related Signaling Pathways in Myelodysplastic Syndrome, Pathology Research and Practice (2024)
Experts (# of related papers)
View all Related Experts
Parvaneh Nikpour (10)
Mohammadreza Sharifi (9)
Other Related Docs
9. Microrna-100 Shuttled by Mesenchymal Stem Cell-Derived Exosomes Suppresses in Vitro Angiogenesis Through Modulating the Mtor/Hif-1Α/Vegf Signaling Axis in Breast Cancer Cells, Cellular Oncology (2017)
10. Circulating Micrornas As Potential Diagnostic Biomarkers and Therapeutic Targets in Gastric Cancer: Current Status and Future Perspectives, Current Medicinal Chemistry (2016)
11. Comprehensive Competitive Endogenous Rna Network Analysis Reveals Ezh2-Related Axes and Prognostic Biomarkers in Hepatocellular Carcinoma, Iranian Journal of Basic Medical Sciences (2022)
12. A Glance at Glioblastoma Molecular Culprits Through In-Silico Analysis, Gene Reports (2021)
13. Identification of Syt10 As a Potential Prognostic Biomarker in Esophageal Cancer by Comprehensive Analysis of a Mrna-Pseudogene/Lncrna-Mirna Cerna Network, Human Gene (2023)
14. Bioinformatics Prediction and Experimental Validation of a Novel Microrna: Hsa-Mir-B43 Within Human Cdh4 Gene With a Potential Metastasis-Related Function in Breast Cancer, Journal of Cellular Biochemistry (2020)
15. Mir-195 Down-Regulation Is a Distinctive Biomarker of Her2 Positive State in Breast Cancer, Gene Reports (2020)
16. Mir-370 Expression Analysis in Breast Cancer, Journal of Isfahan Medical School (2015)
17. The Prominent Role of Mir-942 in Carcinogenesis of Tumors, Advanced Biomedical Research (2022)
18. Apoptosis-Inducing and Antiproliferative Effect by Inhibition of Mir-182-5P Through the Regulation of Casp9 Expression in Human Breast Cancer, Cancer Gene Therapy (2017)
19. Locked Nucleic Acid Anti-Mir-21 Inhibits Cell Growth and Invasive Behaviors of a Colorectal Adenocarcinoma Cell Line: Lna-Anti-Mir As a Novel Approach, Cancer Gene Therapy (2016)
20. Exploring Conserved Mrna-Mirna Interactions in Colon and Lung Cancers, Gastroenterology and Hepatology from Bed to Bench (2017)
21. Evaluation of Silencing Effect of Mir-133A-5P Mimic on Tim-3 Expression in Aml (Hl-60) Cell Line, Indian Journal of Clinical Biochemistry (2020)
22. Mir-196: Emerging of a New Potential Therapeutic Target and Biomarker in Colorectal Cancer, Molecular Biology Reports (2020)
23. The Role of Cancer-Associated Fibroblasts and Exosomal Mirnas-Mediated Intercellular Communication in the Tumor Microenvironment and the Biology of Carcinogenesis: A Systematic Review, Cell Death Discovery (2024)
24. Emerging Biologic and Clinical Implications of Mir-182-5P in Gynecologic Cancers, Clinical and Translational Oncology (2024)
25. Microrna Profiles in Critically Ill Patients, Current Medicinal Chemistry (2024)
26. R0rc2 Gene Silencing in Human Th17 Cells by Sirna: Design and Evaluation of Highly Efficient Sirna, Avicenna Journal of Medical Biotechnology (2013)
27. A Meta-Analysis of Microrna Expression Profiling Studies in Heart Failure, Heart Failure Reviews (2021)
28. The Significant Role of Micrornas in Gliomas Angiogenesis: A Particular Focus on Molecular Mechanisms and Opportunities for Clinical Application, Cellular and Molecular Neurobiology (2023)
29. Deciphering the Multifaceted Role of Micrornas in Hepatocellular Carcinoma: Integrating Literature Review and Bioinformatics Analysis for Therapeutic Insights, Heliyon (2024)
30. Evaluation of the Effect of Tim-3 Suppression by Mir-498 and Its Effect on Apoptosis and Proliferation Rate of Hl-60 Cell Line, Pathology Research and Practice (2018)
31. The Role of Mir-16 and Mir-34A Family in the Regulation of Cancers: A Review, Heliyon (2025)
32. Mir-574, Mir-499, Mir-125B, Mir-106A, and Mir-9 Potentially Target Tgfbr-1 and Tgfbr-2 Genes Involving in Inflammatory Response Pathway: Potential Novel Biomarkers for Chronic Lymphocytic Leukemia, Pathology Research and Practice (2022)
33. Introduction of Lpin1 As a Potential Diagnostic and Prognostic Biomarker for Gastric Cancer Via Integrative Bioinformatics Analysis of a Competing Endogenous Rna Network and Experimental Validation, Iranian Journal of Basic Medical Sciences (2024)
34. Micrornas Regulating Wnt Signaling Pathway in Colorectal Cancer: Biological Implications and Clinical Potentials, Functional and Integrative Genomics (2022)
35. A Review on Expression and Regulatory Mechanisms of Mir-337-3P in Cancer, Journal of Biomolecular Structure and Dynamics (2024)
36. The Mir-125A-3P Inhibits Tim-3 Expression in Aml Cell Line Hl-60 in Vitro, Indian Journal of Hematology and Blood Transfusion (2017)
37. Lna Inhibitor in Microrna Mir-23B As a Potential Anti-Proliferative Option in Human Hepatocellular Carcinoma, Journal of Gastrointestinal Cancer (2020)
38. The Role of Lncrna Mcm3ap-As1 in Human Cancer, Clinical and Translational Oncology (2023)
39. Microrna Binding Site Polymorphism in Inflammatory Genes Associated With Colorectal Cancer: Literature Review and Bioinformatics Analysis, Cancer Gene Therapy (2020)
40. Mir-939, As an Important Regulator in Various Cancers Pathogenesis, Has Diagnostic, Prognostic, and Therapeutic Values: A Review, Journal of the Egyptian National Cancer Institute (2024)
41. Micrornas and Target Molecules in Bladder Cancer, Medical Oncology (2020)
42. Antiproliferative Effect of Upregulation of Hsa-Let-7C-5P in Human Acute Erythroleukemia Cells, Cytotechnology (2018)
43. Msi1 Overexpression in Diffuse Type of Gastric Cancer, Pathology Research and Practice (2013)
44. Micro-Rnas As Critical Regulators of Matrix Metalloproteinases in Cancer, Journal of Cellular Biochemistry (2018)
45. Microrna-196A As a Potential Diagnostic Biomarker for Esophageal Squamous Cell Carcinoma, Cancer Investigation (2017)
46. Micrornas-Mediated Regulation of the Differentiation of Dental Pulp-Derived Mesenchymal Stem Cells: A Systematic Review and Bioinformatic Analysis, Stem Cell Research and Therapy (2023)
47. Deregulation of Mir-1245B-5P and Mir-92A-3P and Their Potential Target Gene, Gata3, in Epithelial–Mesenchymal Transition Pathway in Breast Cancer, Cancer Reports (2024)
48. Regulation of Dna Methylation Machinery by Epi-Mirnas in Human Cancer: Emerging New Targets in Cancer Therapy, Cancer Gene Therapy (2021)
49. Inhibition of Microrna Mir-92A Induces Apoptosis and Inhibits Cell Proliferation in Human Acute Promyelocytic Leukemia Through Modulation of P63 Expression, Molecular Biology Reports (2014)
50. Microrna in Leukemia: Tumor Suppressors and Oncogenes With Prognostic Potential, Journal of Cellular Physiology (2019)