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Immobilization of Lactoperoxidase on Fe3o4 Magnetic Nanoparticles With Improved Stability Publisher Pubmed



Samsam Shariat SZA1 ; Movahedi M2 ; Nazem H2
Authors
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Authors Affiliations
  1. 1. Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Biology, Payame Noor University, P.O. Box, Tehran, 19395-3697, Iran

Source: Biotechnology Letters Published:2019


Abstract

Objective: The study aimed to develop a facile and effectual method to increase the stability of lactoperoxidase (LPO) by using its immobilization on Fe3O4 magnetic nanoparticles (Fe3O4 MNPs). Results: The successful immobilization of LPO on Fe3O4 MNPs was confirmed by using Fourier transform infrared spectroscopy (FT-IR) and field emission scanning electron microscopy (FE-SEM). The Km values of free LPO and LPO immobilized on Fe3O4 were 53.19, 72.46 mM and their Vmax values were 0.629, 0.576 µmol/mL min respectively. The overall results indicated that the stability of the immobilized LPO was significantly improved compared to free LPO. The LPO immobilized on Fe3O4 (LPO– Fe3O4) retained 28% of the initial activity within 30 days at 25 °C whereas the free enzyme lost its activity after 7 days at the same temperature. Moreover, evaluation of the thermal stability of LPO at 75 °C determined the conservation of 19% of the initial activity of LPO in the LPO– Fe3O4 sample after 60 min whereas the free enzyme lost its activity after 5 min. Conclusions: According to the present results, Fe3O4 magnetic nanoparticles are suitable for the immobilization of LPO. © 2019, Springer Nature B.V.
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