Different Liposome Patterns to Detection of Acute Leukemia Based on Electrochemical Cell Sensor Publisher Pubmed



Yazdi MK¹ ; Ghazizadeh E² ; Neshastehriz A^{3, 4} Show Affiliations
Source: Analytica Chimica Acta Published:2020

Abstract

Leukemia is the worst type of malignancy in children which its proper diagnosis can be used in the treatment. We design the turn-off sensor by using the different electrochemical patterns of liposomes to the detection of acute lymphoblastic leukemia cells. Our design is first sandwiched by lectin liposome which increases the electrochemical exchange on the electrode. With the addition of Molt-4 cells, the bonding connection between the n-glycan and lectin can also increase the electrochemical exchange with the high detection cells. Subsequently, the addition of boronic acid liposomes decreases the resistance due to covering glycosylation bond and the sensor is turn-off. But stable and specific binding with the sialic acid causes the higher detection of Molt-4 cells. The electrochemical measurements are performed between the potentials at −0.4 V and +0.4 V with 1 mM [Fe(CN)6] −3/−4. So, for the first time, we designed a cells sensor based on the different patterns of liposomes to screening the N-glycan cells, which can be used in the point of care tests with higher sensitivity. © 2020 Elsevier B.V.