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Gold-Silver Hybrid Nanoparticles As a Novel Carrier for Electrochemical Study of Redox Protein



Eskandari K1 ; Mahheidari N1 ; Fasihiramandi M2 ; Heiat M3 ; Dashtestani F1 ; Kamali M1 ; Ashiani D1
Authors
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Authors Affiliations
  1. 1. Nanobiotechnology Research Center, Baqiyatallah University of Medical Sciences Tehran, Iran
  2. 2. Molecular Biology Research Center, Systems Biology and Poisoning Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
  3. 3. Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Source: Journal of Applied Biotechnology Reports Published:2017

Abstract

Noble metal nanoparticles have a great potential for biological study, especially the use of gold nanoparticles is popular. In this work gold nanoparticles (GNPs), silver nanoparticles (SNPs) and gold-silver hybrid nanoparticles (HNPs) synthesized and used as a carrier for electrochemical investigation of redox protein. Optical characterization of these nanoparticles was performed by UV-Vis spectroscopy. The optical absorption spectra of HNPs solution shows only one plasmon absorption, it is concluded that mixing of gold and silver leads to a homogeneous formation of alloy nanoparticles. LCR meter study shows the HNPs is best conductance in compare of GNPs and SNPs. Therefore, the electron transfer of the homogenous glucose oxidase (GOx), horse radish peroxides (HRP) and hemoglobin (Hb) was investigates by electrochemical method in presence of HNPs. They demonstrated quasi-reversible cyclic voltammograms with a formal potential of-479,-178 and, 168 mV in 50 mM phosphate buffer solution at pH 7.4 respectively. © 2018, Baqiyatallah University of Medical Sciences. All rights reserved