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Thermoresponsive Polyurethane/Siloxane Membrane for Wound Dressing and Cell Sheet Transplantation: In-Vitro and In-Vivo Studies Publisher Pubmed



Rezapourlactoee A1 ; Yeganeh H2 ; Ostad SN1, 3 ; Gharibi R1 ; Mazaheri Z4 ; Ai J1
Authors
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Authors Affiliations
  1. 1. Department of Tissue Engineering, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, 14177-55469, Iran
  2. 2. Iran Polymer and Petrochemical Institute, P.O. Box: 14965/115, Tehran, Iran
  3. 3. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, 16 Azar St, Enqelab Sq, Tehran, 1417614411, Iran
  4. 4. Tarbiat Modares University, Tehran, Iran

Source: Materials Science and Engineering C Published:2016


Abstract

Polyurethane/siloxane based wound dressing for transferring fibroblast cell sheet to wounded skin and ability to provide an optimum condition for cellular activity at damaged tissue was prepared in this research. The dressing was made thermoresponsive, via the introduction of a poly(N-isopropyl acrylamide) copolymer into the backbone of dressing. The ability of membrane for adhesion, growth, and proliferation of fibroblast cells was improved via surface modification with gelatin. The optimized dressing exhibited appropriate tensile strength (4.5 MPa) and elongation at break (80%) to protect wound against physical forces. Due to controlled equilibrium water absorption of about 89% and water vapor transmission rate of 2040 g/m2 day, the dressing could maintain the favorable moist environment over moderate to high exuding wounds. The grown cell sheet on dressing membrane could easily roll up from the surface just with lowering the temperature. The in vivo study of the wound dressed with cell loaded membrane confirmed the accelerated healing and production of tissue with complete re-epithelization, enhanced vascularization, and increased collagen deposition on the damaged area. © 2016 Elsevier B.V.