Increased Expression of Urinary Exosomal Lncrna Tug-1 in Early Bladder Cancer Publisher



Sarfi M^{1, 2} ; Abbastabar M^{1, 2} ; Khalili E¹ Show Affiliations
Source: Gene Reports Published:2021

Abstract

Background: Bladder cancer (BC) is known as one of the most common cancers in the world with high mortality rate. The diagnosis of BC is highly reliable on invasive methods which is not pleasant for patients. Exosomal Long non-coding RNAs (lncRNAs) have been widely studied in the last few years as a potential biomarkers and different studies show pleasing results in this regard. The aim of this study is to evaluate the expression level of two oncogenic lncRNAs (MALAT-1 and TUG-1) in the urinary exosomes of early stages of BC. Material and methods: A total of 40 men enrolled in this study which classified as 30 BC patients and 10 healthy individuals. The fresh urine samples were collected from subjects and after the validation of exosome existence, exosome were extracted. The total RNA of exosomes was extracted base on silica-gel based membrane. Expression level of MALAT-1 and TUG-1 were determined using qRT-PCR. The relationship between their levels and clinicopathological factors of patients with bladder cancer was explored. A receiver operating characteristic (ROC) curve was constructed for differentiating bladder cancer from healthy subjects. Result: TUG-1 and MALAT-1 differential expression levels between tumor and non-tumorous urine samples were 8.7 and 1.4, respectively. There was no significant correlation between the expression level of MALAT-1 and TUG-1 and clinicopathological characteristics. Also no significant difference was found between the expression of lncRNAs among stage I and stage II. Conclusion: Taken together this study has shown that TUG-1 is significantly upregulated in early stages of bladder cancer and could be a potential non-invasive urinary exosome-based biomarker. © 2020 Elsevier Inc.