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Functionalization of Chitosan by Metformin, Nickel Metal Ions and Magnetic Nanoparticles As a Nanobiocomposite for Purification of Alkaline Phosphatase From Hen's Egg Yolk Publisher Pubmed



Eivazzadehkeihan R1 ; Aliabadi HAM2, 3 ; Choopani L1 ; Gorab MG1 ; Rahmati S2 ; Kashtiaray A1 ; Mahdavi M4 ; Maleki A1
Authors
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Authors Affiliations
  1. 1. Department of Chemistry, Catalysts and Organic Synthesis Research Laboratory, Iran University of Science and Technology, Tehran, 16846-13114, Iran
  2. 2. Department of Medical Biotechnology, Protein Chemistry Laboratory, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
  3. 3. Department of Chemistry, Advanced Chemistry Studies Lab, K. N. Toosi University of Technology, Tehran, Iran
  4. 4. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Chromatography A Published:2022


Abstract

In this study, a novel and green chitosan-metformin/NiCl2/Fe3O4 nanobiocomposite was synthesized and used to purify alkaline phosphatase (ALPs) from hen's egg yolk. For this purpose, after functionalization of the chitosan biopolymer by terephthaloyl chloride-metformin ligand, the coordination with Ni(II) and magnetization process were performed. The structure and properties of the synthesized nanobiocomposite were then evaluated by using analyzes such as FT-IR, EDX, FE-SEM, XRD, TGA and VSM. Purification of ALPs with chitosan-metformin/NiCl2/Fe3O4 nanobiocomposite is a fast, reusable and cost-effective method. By this protocol, 62% purification efficiency was obtained and the synthesized nanobiocomposite was not attached to other proteins in hen's egg yolk. ALPs was obtained approximately in the pure form and the purification process was evaluated using SDS-PAGE. The reusability of nanobiocomposites was evaluated and a slight decrease in adsorption capacity was observed after 4 cycles. © 2022
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