Chitosan/Silk Fibroin/Nitrogen-Doped Carbon Quantum Dot/Α-Tricalcium Phosphate Nanocomposite Electrospinned As a Scaffold for Wound Healing Application: In Vitro and in Vivo Studies

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Chitosan/Silk Fibroin/Nitrogen-Doped Carbon Quantum Dot/Α-Tricalcium Phosphate Nanocomposite Electrospinned As a Scaffold for Wound Healing Application: In Vitro and in Vivo Studies Publisher Pubmed

Dehghani N^{1, 2, 3} ; Haghiralsadat F⁴ ; Yazdian F⁵ ; Sadeghiannodoushan F^{6, 7} ; Ghasemi N⁸ ; Mazaheri F^{9, 10} ; Pourmadadi M¹¹ ; Naghib SM¹²

Show Affiliations

1. Department of Genetics, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
2. Department of Medical Biotechnology, School of Medicine, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
3. Biomaterials and Tissue Engineering Research Group, Interdisciplinary Technologies Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
4. Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
5. Department of Life Science Engineering, Faculty of New Science and Technology, Tehran, Iran
6. Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
7. Medical Nanotechnology & Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
8. Abortion Research Center, Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran
9. Medical Nanotechnology and Tissue Engineering Research Centre, Yazd Reproductive Science Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
10. Research and Clinical Center of Infertility, Yazd Reproductive Science Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
11. Protein Research Center, Shahid Beheshti University, GC, Tehran, Iran
12. Nanotechnology Department, School of Advanced Technologies, Iran University of Science and Technology, Tehran, Iran

Source: International Journal of Biological Macromolecules Published:2023

Abstract

A highly porous nanofibrous network that can functionalize antibacterial and therapeutic agents can be considered a suitable option for skin wound healing. In this study, α-tricalcium phosphate (α-TCP)/nitrogen-doped carbon quantum dots (N-CQDs) nanocomposite was synthesized and then applied to the fabrication of novel chitosan (CS)/silk fibroin (SF)/N-CQDs/α-TCP wound dressing via electrospinning system. The prepared nanomaterials were well characterized using X-ray diffraction, Fourier-transform infrared, scanning and transmission electron microscopes analyses, and antibacterial assay. Furthermore, nanofibers were evaluated regarding their physical properties, such as tensile behavior, water uptake capacity, and water contact angle. The results reveal that CS/SF/N-CQDs/α-TCP showed lower MIC values against E. coli and S. aureus (1.45 ± 0.26 mg/mL and 1.59 ± 0.12 mg/mL) compared to other synthesized materials. Also, in-vitro investigations were performed, and the MTT assay on the HFF cell line revealed that CS/SF/N-CQDs/α-TCP nanofiber could possess good biocompatibility. Interestingly, the scratch test proved that faster cell migration and proliferation occurred in the presence of CS/SF/N-CQDs/α-TCP 73.23 ± 2.71 %). Finally, we examined the wound healing ability of CS/SF/N-CQDs/α-TCP nanofiber using an animal model. The results confirmed that produced nanofiber could efficiently promote wound closure by 96.73 ± 1.25 % in 12 days. Histopathological analyses verified accelerated re-epithelization and well-structured epidermis in CS/SF/N-CQDs/α-TCP nanofiber-treated group. Based on our findings, the CS/SF/N-CQDs/α-TCP nanofiber with excellent antimicrobial properties is highly suitable for wound healing and skin tissue regeneration applications. © 2023 Elsevier B.V.

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Style	Citing Format
MLA	Dehghani N, et al.. "Chitosan/Silk Fibroin/Nitrogen-Doped Carbon Quantum Dot/Α-Tricalcium Phosphate Nanocomposite Electrospinned As a Scaffold for Wound Healing Application: In Vitro and in Vivo Studies." International Journal of Biological Macromolecules, vol. 238, no. , 2023, pp. -.
APA	Dehghani N, Haghiralsadat F, Yazdian F, Sadeghiannodoushan F, Ghasemi N, Mazaheri F, Pourmadadi M, Naghib SM (2023). Chitosan/Silk Fibroin/Nitrogen-Doped Carbon Quantum Dot/Α-Tricalcium Phosphate Nanocomposite Electrospinned As a Scaffold for Wound Healing Application: In Vitro and in Vivo Studies. International Journal of Biological Macromolecules, 238(), -.
Chicago	Dehghani N, Haghiralsadat F, Yazdian F, Sadeghiannodoushan F, Ghasemi N, Mazaheri F, Pourmadadi M, Naghib SM. "Chitosan/Silk Fibroin/Nitrogen-Doped Carbon Quantum Dot/Α-Tricalcium Phosphate Nanocomposite Electrospinned As a Scaffold for Wound Healing Application: In Vitro and in Vivo Studies." International Journal of Biological Macromolecules 238, no. (2023): -.
Harvard	Dehghani N et al. (2023) 'Chitosan/Silk Fibroin/Nitrogen-Doped Carbon Quantum Dot/Α-Tricalcium Phosphate Nanocomposite Electrospinned As a Scaffold for Wound Healing Application: In Vitro and in Vivo Studies', International Journal of Biological Macromolecules, 238(), pp. -.
Vancouver	Dehghani N, Haghiralsadat F, Yazdian F, Sadeghiannodoushan F, Ghasemi N, Mazaheri F, et al.. Chitosan/Silk Fibroin/Nitrogen-Doped Carbon Quantum Dot/Α-Tricalcium Phosphate Nanocomposite Electrospinned As a Scaffold for Wound Healing Application: In Vitro and in Vivo Studies. International Journal of Biological Macromolecules. 2023;238():-.
BibTex	@article{ author = {Dehghani N and Haghiralsadat F and Yazdian F and Sadeghiannodoushan F and Ghasemi N and Mazaheri F and Pourmadadi M and Naghib SM}, title = {Chitosan/Silk Fibroin/Nitrogen-Doped Carbon Quantum Dot/Α-Tricalcium Phosphate Nanocomposite Electrospinned As a Scaffold for Wound Healing Application: In Vitro and in Vivo Studies}, journal = {International Journal of Biological Macromolecules}, volume = {238}, number = {}, pages = {-}, year = {2023} }
RIS	TY - JOUR AU - Dehghani N AU - Haghiralsadat F AU - Yazdian F AU - Sadeghiannodoushan F AU - Ghasemi N AU - Mazaheri F AU - Pourmadadi M AU - Naghib SM TI - Chitosan/Silk Fibroin/Nitrogen-Doped Carbon Quantum Dot/Α-Tricalcium Phosphate Nanocomposite Electrospinned As a Scaffold for Wound Healing Application: In Vitro and in Vivo Studies JO - International Journal of Biological Macromolecules VL - 238 IS - SP - EP - PY - 2023 ER -

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