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Evaluation of Antimicrobial Effects of Photo-Sonodynamic Antimicrobial Chemotherapy Based on Nano-Micelle Curcumin on Virulence Gene Expression Patterns in Acinetobacter Baumannii Publisher Pubmed



Pourhajibagher M1 ; Talaei N2 ; Bahador A3, 4
Authors
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Authors Affiliations
  1. 1. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran
  3. 3. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Fellowship in Clinical Laboratory Sciences, Iran University of Medical Sciences, Tehran, Iran

Source: Infectious Disorders - Drug Targets Published:2022


Abstract

Background: Abaumannii baumannii rapidly resistant to a wide range of antimicrobial agents. The combination of Antimicrobial Photodynamic Therapy (aPDT) and Sonodynamic Antimi-crobial Chemotherapy (SACT), known as Photo-Sonodynamic Antimicrobial Chemotherapy (PSACT), has received considerable attention as one of the emerging and promising strategies against microbial infections. Objective: This study aimed to investigate the antimicrobial effects of PSACT based on nano-micelle curcumin (N-MCur) on the virulence gene expression patterns in A. baumannii. Materials and Methods: N-MCur as a photo-sonosensitizer was synthesized and confirmed. To deter-mine sub-significant reduction dose of PSACT, sub-significant reduction dose of N-MCur and blue laser light during aPDT, and ultrasound power output during SACT was assessed. Finally, changes in the expression of genes involved in treated A. baumannii by minimum sub-significant reduction dose of PSACT were determined using quantitative real-time-PCR (qRT-PCR). Results: PSACT using 12.5 mM N-MCur at the ultrasound power outputs of 28.7, 36.9, and 45.2 mW/cm2 with 4 min irradiation time of the blue laser, as well as 6.2 mM N-MCur at an ultrasound power output of 45.2 mW/cm2 plus 3 min blue laser irradiation time exhibited the significant dose-dependent reduction against A. baumannii cell viability compared to the control group (P<0.05). After treatment of A. baumannii using 3.1 mM N-MCur + 2 min blue laser irradiation time + 28.7 mW/cm2 ultrasound as the minimum sub-significant reduction doses of PSACT, mRNA expression was signifi-cantly upregulated to 6.0-, 11.2-, and 13.7-folds in recA, blsA, and dnaK and downregulated to 8.6-, 10.1-, and 14.5-folds in csuE, espA, and abaI, respectively. Conclusion: N-MCur-mediated PSACT could regulate the expression of genes involved in A. bau-mannii pathogenesis. Therefore, PSACT can be proposed as a promising application to treat infections caused by A. baumannii. © 2022 Bentham Science Publishers.
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