Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Dna-Aptamer-Nanographene Oxide As a Targeted Bio-Theragnostic System in Antimicrobial Photodynamic Therapy Against Porphyromonas Gingivalis Publisher Pubmed



Pourhajibagher M1 ; Etemadmoghadam S1 ; Alaeddini M1 ; Miri Mousavi R2 ; Bahador A1, 3
Authors
Show Affiliations
Authors Affiliations
  1. 1. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Pharmaceutical Engineering Laboratory, School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran
  3. 3. Fellowship in Clinical Laboratory Sciences, BioHealth Lab, Tehran, Iran

Source: Scientific Reports Published:2022


Abstract

The aim of this study was to design and evaluate the specificity of a targeted bio-theragnostic system based on DNA-aptamer-nanographene oxide (NGO) against Porphyromonas gingivalis during antimicrobial photodynamic therapy (aPDT). Following synthesis and confirmation of NGO, the binding of selected labeled DNA-aptamer to NGO was performed and its hemolytic activity, cytotoxic effect, and release times were evaluated. The specificity of DNA-aptamer-NGO to P. gingivalis was determined. The antimicrobial effect, anti-biofilm potency, and anti-metabolic activity of aPDT were then assessed after the determination of the bacteriostatic and bactericidal concentrations of DNA-aptamer-NGO against P. gingivalis. Eventually, the apoptotic effect and anti-virulence capacity of aPDT based on DNA-aptamer-NGO were investigated. The results showed that NGO with a flaky, scale-like, and layered structure in non-cytotoxic DNA-aptamer-NGO has a continuous release in the weak-acid environment within a period of 240 h. The binding specificity of DNA-aptamer-NGO to P. gingivalis was confirmed by flow cytometry. When irradiated, non-hemolytic DNA-aptamer-NGO were photoactivated, generated ROS, and led to a significant decrease in the cell viability of P. gingivalis (P < 0.05). Also, the data indicated that DNA-aptamer-NGO-mediated aPDT led to a remarkable reduction of biofilms and metabolic activity of P. gingivalis compared to the control group (P < 0.05). In addition, the number of apoptotic cells increased slightly (P > 0.05) and the expression level of genes involved in bacterial biofilm formation and response to oxidative stress changed significantly after exposure to aPDT. It is concluded that aPDT using DNA-aptamer-NGO as a targeted bio-theragnostic system is a promising approach to detect and eliminate P. gingivalis as one of the main bacteria involved in periodontitis in periopathogenic complex in real-time and in situ. © 2022, The Author(s).
Related Docs
View other Related Docs
1. In Vitro Antibacterial Activity of Photoactivated Flavonoid Glycosides Against Acinetobacter Baumannii, AMB Express (2024)
2. Theranostic Nanoplatforms of Emodin-Chitosan With Blue Laser Light on Enhancing the Anti-Biofilm Activity of Photodynamic Therapy Against Streptococcus Mutans Biofilms on the Enamel Surface, BMC Microbiology (2022)
3. Changes of Microbial Cell Survival, Metabolic Activity, Efflux Capacity, and Quorum Sensing Ability of Aggregatibacter Actinomycetemcomitans Due to Antimicrobial Photodynamic Therapy-Induced Bystander Effects, Photodiagnosis and Photodynamic Therapy (2019)
4. Expression Patterns of Oxyr Induced by Oxidative Stress From Porphyromonas Gingivalis in Response to Photo-Activated Disinfection, Infection and Drug Resistance (2018)
5. Therapeutic Applications of Nucleic Acid Aptamers in Microbial Infections, Journal of Biomedical Science (2020)
6. Real-Time Quantitative Reverse Transcription-Pcr Analysis of Expression Stability of Aggregatibacter Actinomycetemcomitans Fimbria-Associated Gene in Response to Photodynamic Therapy, Photodiagnosis and Photodynamic Therapy (2017)
7. Potentiation Effects of Antimicrobial Photodynamic Therapy on Quorum Sensing Genes Expression: A Promising Treatment for Multi-Species Bacterial Biofilms in Burn Wound Infections, Photodiagnosis and Photodynamic Therapy (2020)
8. Quorum Quenching of Streptococcus Mutans Via the Nano-Quercetin-Based Antimicrobial Photodynamic Therapy As a Potential Target for Cariogenic Biofilm, BMC Microbiology (2022)
Experts (# of related papers)
View all Related Experts
Maryam Pourhajibagher (29)
Abbas Bahador (29)
Other Related Docs
9. Antimicrobial Activity of D-Amino Acid in Combination With Photo-Sonoactivated Hypericin Nanoparticles Against Acinetobacter Baumannii, BMC Microbiology (2023)
10. Physico-Mechanical and Antimicrobial Properties of an Elastomeric Ligature Coated With Reduced Nanographene Oxide-Nano Curcumin Subjected to Dual-Modal Photodynamic and Photothermal Inactivation Against Streptococcus Mutans Biofilms, Photodiagnosis and Photodynamic Therapy (2023)
11. Anti-Virulence Effect of Photoactivated Nano-Quercetin by Diode Laser on Aggregatibacter Actinomycetemcomitans, AMB Express (2025)
12. Synergistic Effects of Nano Curcumin Mediated Photodynamic Inactivation and Nano-Silver@Colistin Against Pseudomonas Aeruginosa Biofilms, Photodiagnosis and Photodynamic Therapy (2024)
13. Application of Photosensitive Dental Materials As a Novel Antimicrobial Option in Dentistry: A Literature Review, Journal of Dental Sciences (2024)
14. The Anti-Biofilm Capability of Nano-Emodin-Mediated Sonodynamic Therapy on Multi-Species Biofilms Produced by Burn Wound Bacterial Strains, Photodiagnosis and Photodynamic Therapy (2021)
15. Periodontal Ligament Stem Cell-Derived Exosome-Loaded Emodin Mediated Antimicrobial Photodynamic Therapy Against Cariogenic Bacteria, BMC Oral Health (2024)
16. Monitoring Gene Expression of Rcpa From Aggregatibacter Actinomycetemcomitans Versus Antimicrobial Photodynamic Therapy by Relative Quantitative Real-Time Pcr, Photodiagnosis and Photodynamic Therapy (2017)
17. Exploring Photoactivated Disinfection-Induced Bystander Effects on Microbial Biofilms of Aggregatibacter Actinomycetemcomitans, Infectious Disorders - Drug Targets (2021)
18. Anti-Multispecies Microbial Biofilms and Anti-Inflammatory Effects of Antimicrobial Photo-Sonodynamic Therapy Based on Acrylic Resin Containing Nano-Resveratrol, Photodiagnosis and Photodynamic Therapy (2023)
19. Graphene-Based Polymer Nanocomposites: Chemistry and Applications, Advanced Structured Materials (2015)
20. Types of Rna Therapeutics, Progress in Molecular Biology and Translational Science (2024)
21. Evaluation of Antimicrobial Effects of Photo-Sonodynamic Antimicrobial Chemotherapy Based on Nano-Micelle Curcumin on Virulence Gene Expression Patterns in Acinetobacter Baumannii, Infectious Disorders - Drug Targets (2022)
22. Investigation of Arginine A-Specific Cysteine Proteinase Gene Expression Profiling in Clinical Porphyromonas Gingivalis Isolates Against Photokilling Action of the Photo-Activated Disinfection, Lasers in Medical Science (2018)
23. The Effect of Antimicrobial Photodynamic Therapy on the Expression of Biofilm Associated Genes In Staphylococcus Aureus Strains Isolated From Wound Infections in Burn Patients, Photodiagnosis and Photodynamic Therapy (2019)
24. Contribution of Antimicrobial Photo-Sonodynamic Therapy in Wound Healing: An in Vivo Effect of Curcumin-Nisin-Based Poly (L-Lactic Acid) Nanoparticle on Acinetobacter Baumannii Biofilms, BMC Microbiology (2022)
25. Photoelimination Potential of Chitosan Nanoparticles-Indocyanine Green Complex Against the Biological Activities of Acinetobacter Baumannii Strains: A Preliminary in Vitro Study in Burn Wound Infections, Journal of Lasers in Medical Sciences (2020)
26. Photodisinfection Effects of Silver Sulfadiazine Nanoliposomes Doped-Curcumin on Acinetobacter Baumannii: A Mouse Model, Nanomedicine (2020)
27. Immobilization of L-Asparaginase on Aspartic Acid Functionalized Graphene Oxide Nanosheet: Enzyme Kinetics and Stability Studies, Chemical Engineering Journal (2018)
28. Graphene Oxide Nanoarchitectures in Cancer Therapy: Drug and Gene Delivery, Phototherapy, Immunotherapy, and Vaccine Development, Environmental Research (2023)
29. Anti-Biofilm and Bystander Effects of Antimicrobial Photo-Sonodynamic Therapy Against Polymicrobial Periopathogenic Biofilms Formed on Coated Orthodontic Mini-Screws With Zinc Oxide Nanoparticles, Photodiagnosis and Photodynamic Therapy (2023)
30. Application of Graphene Materials in Molecular Diagnostics, Handbook of Graphene (2019)
31. The Effect of Antimicrobial Photodynamic Therapy on the Expression of Novel Methicillin Resistance Markers Determined Using Cdna-Aflp Approach in Staphylococcus Aureus, Photodiagnosis and Photodynamic Therapy (2017)
32. The Effects of Bimodal Action of Photodynamic and Photothermal Therapy on Antimicrobial and Shear Bond Strength Properties of Orthodontic Composite Containing Nano-Graphene Oxide, Photodiagnosis and Photodynamic Therapy (2021)
33. Hb5 Aptamer-Tagged Graphene Oxide for Co-Delivery of Doxorubicin and Silibinin, and Highly Effective Combination Therapy in Breast Cancer, Cancer Nanotechnology (2023)
34. Attenuation of Aggregatibacter Actinomycetemcomitans Virulence Using Curcumin-Decorated Nanophytosomes-Mediated Photo-Sonoantimicrobial Chemotherapy, Scientific Reports (2021)
35. Effects of 8-Hydroxyquinoline-Coated Graphene Oxide on Cell Death and Apoptosis in Mcf-7 and Mcf-10 Breast Cell Lines, Iranian Journal of Basic Medical Sciences (2020)
36. Modulation of Virulence in Enterococcus Faecalis Cells Surviving Antimicrobial Photodynamic Inactivation With Reduced Graphene Oxide-Curcumin: An Ex Vivo Biofilm Model, Photodiagnosis and Photodynamic Therapy (2020)
37. Carnosine-Graphene Oxide Conjugates Decorated With Hydroxyapatite As Promising Nanocarrier for Icg Loading With Enhanced Antibacterial Effects in Photodynamic Therapy Against Streptococcus Mutans, Journal of Photochemistry and Photobiology B: Biology (2018)
38. The Effect of Sublethal Photodynamic Therapy on the Expression of Enterococcal Surface Protein (Esp) Encoding Gene in Enterococcus Faecalis: Quantitative Real-Time Pcr Assessment, Photodiagnosis and Photodynamic Therapy (2018)