Electrospun Cellulose Acetate/Gelatin Nanofibrous Wound Dressing Containing Berberine for Diabetic Foot Ulcer Healing: In Vitro and in Vivo Studies

Electrospun Cellulose Acetate/Gelatin Nanofibrous Wound Dressing Containing Berberine for Diabetic Foot Ulcer Healing: In Vitro and in Vivo Studies Publisher Pubmed

Samadian H¹ ; Zamiri S² ; Ehterami A³ ; Farzamfar S⁴ ; Vaez A⁵ ; Khastar H⁶ ; Alam M⁷ ; Ai A⁸ ; Derakhshankhah H¹ ; Allahyari Z^{9, 10} ; Goodarzi A¹¹ ; Salehi M^{12, 13}

Show Affiliations

1. Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
2. Department of Kinesiology and Health Science, York University, ON, Canada
3. Department of Mechanical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
4. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
5. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
6. School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
7. Department of Oral and Maxillofacial Surgery, Dental School, Shahid Beheshti University of Medical sciences, Tehran, Iran
8. Dental student of scientific research center, faculty of dentistry, Tehran university of medical sciences, Tehran, Iran
9. Department of Biomedical Engineering, Rochester Institute of Technology, Rochester, United States
10. Department of Microsystems Engineering, Rochester Institute of Technology, Rochester, NY, United States
11. Department of Tissue Engineering, School of Advanced Technologies, Fasa University of Medical Sciences, Fasa, Iran
12. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
13. Tissue Engineering and stem cells research center, Shahroud University of Medical Sciences, Shahroud, Iran

Source: Scientific Reports Published:2020

Abstract

Functional wound dressing with tailored physicochemical and biological properties is vital for diabetic foot ulcer (DFU) treatment. Our main objective in the current study was to fabricate Cellulose Acetate/Gelatin (CA/Gel) electrospun mat loaded with berberine (Beri) as the DFU-specific wound dressing. The wound healing efficacy of the fabricated dressings was evaluated in streptozotocin-induced diabetic rats. The results demonstrated an average nanofiber diameter of 502 ± 150 nm, and the tensile strength, contact angle, porosity, water vapor permeability and water uptake ratio of CA/Gel nanofibers were around 2.83 ± 0.08 MPa, 58.07 ± 2.35°, 78.17 ± 1.04%, 11.23 ± 1.05 mg/cm2/hr, and 12.78 ± 0.32%, respectively, while these values for CA/Gel/Beri nanofibers were 2.69 ± 0.05 MPa, 56.93 ± 1°, 76.17 ± 0.76%, 10.17 ± 0.21 mg/cm2/hr, and 14.37 ± 0.42%, respectively. The antibacterial evaluations demonstrated that the dressings exhibited potent antibacterial activity. The collagen density of 88.8 ± 6.7% and the angiogenesis score of 19.8 ± 3.8 obtained in the animal studies indicate a proper wound healing. These findings implied that the incorporation of berberine did not compromise the physical properties of dressing, while improving the biological activities. In conclusion, our results indicated that the prepared mat is a proper wound dressing for DFU management and treatment. © 2020, The Author(s).

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Style	Citing Format
MLA	Samadian H, et al.. "Electrospun Cellulose Acetate/Gelatin Nanofibrous Wound Dressing Containing Berberine for Diabetic Foot Ulcer Healing: In Vitro and in Vivo Studies." Scientific Reports, vol. 10, no. 1, 2020, pp. -.
APA	Samadian H, Zamiri S, Ehterami A, Farzamfar S, Vaez A, Khastar H, Alam M, Ai A, Derakhshankhah H, Allahyari Z, Goodarzi A, Salehi M (2020). Electrospun Cellulose Acetate/Gelatin Nanofibrous Wound Dressing Containing Berberine for Diabetic Foot Ulcer Healing: In Vitro and in Vivo Studies. Scientific Reports, 10(1), -.
Chicago	Samadian H, Zamiri S, Ehterami A, Farzamfar S, Vaez A, Khastar H, Alam M, et al.. "Electrospun Cellulose Acetate/Gelatin Nanofibrous Wound Dressing Containing Berberine for Diabetic Foot Ulcer Healing: In Vitro and in Vivo Studies." Scientific Reports 10, no. 1 (2020): -.
Harvard	Samadian H et al. (2020) 'Electrospun Cellulose Acetate/Gelatin Nanofibrous Wound Dressing Containing Berberine for Diabetic Foot Ulcer Healing: In Vitro and in Vivo Studies', Scientific Reports, 10(1), pp. -.
Vancouver	Samadian H, Zamiri S, Ehterami A, Farzamfar S, Vaez A, Khastar H, et al.. Electrospun Cellulose Acetate/Gelatin Nanofibrous Wound Dressing Containing Berberine for Diabetic Foot Ulcer Healing: In Vitro and in Vivo Studies. Scientific Reports. 2020;10(1):-.
BibTex	@article{ author = {Samadian H and Zamiri S and Ehterami A and Farzamfar S and Vaez A and Khastar H and Alam M and Ai A and Derakhshankhah H and Allahyari Z and Goodarzi A and Salehi M}, title = {Electrospun Cellulose Acetate/Gelatin Nanofibrous Wound Dressing Containing Berberine for Diabetic Foot Ulcer Healing: In Vitro and in Vivo Studies}, journal = {Scientific Reports}, volume = {10}, number = {1}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Samadian H AU - Zamiri S AU - Ehterami A AU - Farzamfar S AU - Vaez A AU - Khastar H AU - Alam M AU - Ai A AU - Derakhshankhah H AU - Allahyari Z AU - Goodarzi A AU - Salehi M TI - Electrospun Cellulose Acetate/Gelatin Nanofibrous Wound Dressing Containing Berberine for Diabetic Foot Ulcer Healing: In Vitro and in Vivo Studies JO - Scientific Reports VL - 10 IS - 1 SP - EP - PY - 2020 ER -

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