Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Different Osteogenic Differentiation Potential of Mesenchymal Stem Cells on Three Different Polymeric Substrates Publisher Pubmed



Abazari MF1 ; Hosseini Z2 ; Zare Karizi S3 ; Norouzi S4 ; Amini Faskhoudi M5 ; Saburi E6 ; Enderami SE7 ; Ardeshirylajimi A8 ; Mohajerani H9
Authors
Show Affiliations
Authors Affiliations
  1. 1. Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Biology, Kharazmi University, Tehran, Iran
  3. 3. Department of Biology, Varamin Pishva Branch, Islamic Azad University, Pishva, Varamin, Iran
  4. 4. Department of Biology, Faculty of Science and Research, Islamic Azad University, Tehran, Iran
  5. 5. Department of Biology, Tehran Medical Branch, Islamic Azad University, Tehran, Iran
  6. 6. Medical Genetics and Molecular Medicine Department, Mashhad University of Medical Sciences, Mashhad, Iran
  7. 7. Immunogenetics Research Center, Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  8. 8. Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University, St. Louis, MO, United States
  9. 9. Department of Oral & Maxillofacial Surgery, Dental School, Shahid Beheshti University Of Medical Sciences, Tehran, Iran

Source: Gene Published:2020


Abstract

The function of tissue cells is strongly depends on the extracellular matrix (ECM) that can guide and support cell structure. This support plays a crucial role in the process of cell proliferation and differentiation. Herein, three different nanofibrous scaffolds that are highly attractive for tissue engineering were selected and then osteogenic related genes and protein expression patterns of human adipose-derived mesenchymal stem cells (AT-MSCs) were investigated when grown on substrates. Polycaprolactone, Poly (L-lactic acid) and Polyvinylidene-fluoride nanofibrous scaffolds were fabricated using Electrospinning method and then AT-MSCs viability and osteogenic differentiation were evaluated while cultured on them. The highest AT-MSCs survival rate when grown on the scaffolds was detected when grown on Polyvinylidene-fluoride. In addition, the highest ALP activity and mineralization were also observed in differentiated AT-MSCs has grown on Polyvinylidene-fluoride. The expression levels of Runx2, osteonectin and osteocalcin genes and osteocalcin protein in the AT-MSCs has grown on the Polyvinylidene-fluoride were also significantly higher than the rest of the scaffolds. Based on the results, it seems that since the studied substrate have a similar structural characteristics, their nature may have an important role in the stem cell's osteogenesis process, where the Polyvinylidene-fluoride piezoelectricity was a most distinguished characteristic. © 2020 Elsevier B.V.
Related Docs
View other Related Docs
1. Improved Osteogenic Differentiation of Human Induced Pluripotent Stem Cells Cultured on Polyvinylidene Fluoride/Collagen/Platelet-Rich Plasma Composite Nanofibers, Journal of Cellular Physiology (2020)
2. Bone Tissue Engineering: Adult Stem Cells in Combination With Electrospun Nanofibrous Scaffolds, Journal of Cellular Physiology (2018)
3. Poly (Glycerol Sebacate) and Polyhydroxybutyrate Electrospun Nanocomposite Facilitates Osteogenic Differentiation of Mesenchymal Stem Cells, Journal of Drug Delivery Science and Technology (2021)
4. Comparison of Osteogenic Differentiation Potential of Induced Pluripotent Stem Cells on 2D and 3D Polyvinylidene Fluoride Scaffolds, Journal of Cellular Physiology (2019)
5. Functionalized Silk Fibroin Nanofibers As Drug Carriers: Advantages and Challenges, Journal of Controlled Release (2020)
6. Poly (3-Hydroxybutyrate-Co-3-Hydroxyvalerate) Improved Osteogenic Differentiation of the Human Induced Pluripotent Stem Cells While Considered As an Artificial Extracellular Matrix, Journal of Cellular Physiology (2019)
7. The Role of Nanomaterials in Cell Delivery Systems, Medical Molecular Morphology (2018)
8. A Novel Silk/Pes Hybrid Nanofibrous Scaffold Promotes the in Vitro Proliferation and Differentiation of Adipose-Derived Mesenchymal Stem Cells Into Insulin Producing Cells, Polymers for Advanced Technologies (2020)
Experts (# of related papers)
View all Related Experts
Nima Rezaei (1)
Mehdi Norouzi (1)
Other Related Docs
9. Microrna-2861 and Nanofibrous Scaffold Synergistically Promote Human Induced Pluripotent Stem Cells Osteogenic Differentiation, Polymers for Advanced Technologies (2020)
10. Efficient Osteogenic Differentiation of the Dental Pulp Stem Cells on Β-Glycerophosphate Loaded Polycaprolactone/Polyethylene Oxide Blend Nanofibers, Journal of Cellular Physiology (2019)
11. Biologically Modified Electrospun Polycaprolactone Nanofibrous Scaffold Promotes Osteogenic Differentiation, Journal of Drug Delivery Science and Technology (2022)
12. The Clinical Trials of Mesenchymal Stem Cell Therapy in Skin Diseases: An Update and Concise Review, Current Stem Cell Research and Therapy (2019)
13. In Vitro Fibroblast Migration by Sustained Release of Pdgf-Bb Loaded in Chitosan Nanoparticles Incorporated in Electrospun Nanofibers for Wound Dressing Applications, Artificial Cells# Nanomedicine and Biotechnology (2018)
14. Promoted Osteogenic Differentiation of Human Induced Pluripotent Stem Cells Using Composited Polycaprolactone/Polyvinyl Alcohol/Carbopol Nanofibrous Scaffold, Journal of Drug Delivery Science and Technology (2022)
15. Micro-Rna-Incorporated Electrospun Nanofibers Improve Osteogenic Differentiation of Human-Induced Pluripotent Stem Cells, Journal of Biomedical Materials Research - Part A (2020)
16. Electrospun Fibers: Versatile Approaches for Controlled Release Applications, International Journal of Polymer Science (2022)
17. Improved Chondrogenic Response of Mesenchymal Stem Cells to a Polyethersulfone/Polyaniline Blended Nanofibrous Scaffold, Journal of Cellular Biochemistry (2019)
18. In Vitro Osteogenic Differentiation of Stem Cells With Different Sources on Composite Scaffold Containing Natural Bioceramic and Polycaprolactone, Artificial Cells# Nanomedicine and Biotechnology (2019)
19. Curcumin-Loaded Phb/Plla Nanofibrous Scaffold Supports Osteogenesis in Adipose-Derived Stem Cells in Vitro, Polymers for Advanced Technologies (2021)
20. Nanomedicine and Advanced Technologies for Burns: Preventing Infection and Facilitating Wound Healing, Advanced Drug Delivery Reviews (2018)
21. Synergistic Effects of Polyaniline and Pulsed Electromagnetic Field to Stem Cells Osteogenic Differentiation on Polyvinylidene Fluoride Scaffold, Artificial Cells# Nanomedicine and Biotechnology (2019)
22. Commercialization and Regulation of Regenerative Medicine Products: Promises, Advances and Challenges, Biomedicine and Pharmacotherapy (2022)
23. Platelet-Rich Plasma Incorporated Electrospun Pva-Chitosan-Ha Nanofibers Accelerates Osteogenic Differentiation and Bone Reconstruction, Gene (2019)
24. Incorporated-Bfgf Polycaprolactone/Polyvinylidene Fluoride Nanocomposite Scaffold Promotes Human Induced Pluripotent Stem Cells Osteogenic Differentiation, Journal of Cellular Biochemistry (2019)