Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Bulge Hair Follicle Stem Cells Accelerate Cutaneous Wound Healing in Rats Pubmed



Heidari F1, 2 ; Yari A3 ; Rasoolijazi H1 ; Soleimani M1, 4 ; Dehpoor A5 ; Sajedi N1 ; Joulai Veijouye S1 ; Nobakht M1, 6, 7
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomy, School of Medicine, Iran University of Medical Science, Hemmat Highway, Tehran, 6183, Iran
  2. 2. Department of Anatomy, School of Medicine, Qom University of Medical Science, Qom, Iran
  3. 3. Department of Anatomy, School of Medicine, Alborz University of Medical Science, Karaj, Iran
  4. 4. Cellular and Molecular Research Center, Faculty of Medicine, Iran University of Medical Science, Tehran, Iran
  5. 5. Department of Pharmacology, School of Medicine, Tehran University of Medical Science, Tehran, Iran
  6. 6. Physiology Research Center, Iran University of Medical Science, Tehran, Iran
  7. 7. Anti-Microbial Resistance Research Center, Iran University of Medical Science, Tehran, Iran

Source: Wounds Published:2016


Abstract

Objective. Skin wound healing is a serious clinical problem especially after surgery and severe injury of the skin. Cell therapy is an innovative technique that can be applied to wound healing. One appropriate source of stem cells for therapeutic use is stem cells from the adult bulge of hair follicles. This study examined the effects of adult bulge hair follicle stem cells (HFSC) in wound healing. Materials and Methods. Hair follicle stem cells were ob- tained from rat vibrissa and labeled with DiI (Invitrogen, Carlsbad, CA), then special markers were detected using flow cytometry. A full-thickness excisional wound model was created and DiI-labeled HFSC were injected around the wound bed. Wound healing was re- corded with digital photographs. Animals were sacrificed at 3, 7, or 14 days after surgery, and were used for the following histological analyses. Results. Flow cytometry analysis showed that HFSC were CD34 positive and nestin positive, but K15 negative. Morphologi- cal analysis of HFSC-treated wounds exhibited accelerated wound closure. Histological analysis of hematoxylin and eosin stained and Massongs trichrome-stained photomicrographs showed significantly more re-epithelialization and dermal structural regeneration in HF- SC-treated wounds than in the control group. Immunohistochemical analysis of CD31 protein-positive cells showed angiogenesis was also more significant in HFSC-treated wounds than in the control group. Conclusion. Hair follicle stem cells accelerate skin wound healing. Isolating HFSC from a small skin biopsy could repair less- extensive full-thickness skin wounds by autologous stem cells and overcome major challenges regarding the use of stem cells in clini- cal application, while avoiding immune rejection and ethical concerns.
Related Docs
1. Hair Follicle Stem Cells Promote Cutaneous Wound Healing Through the Sdf-1A/Cxcr4 Axis: An Animal Model, Journal of Wound Care (2020)
2. In Vitro Induction Effect of 1,25(Oh)2D3 on Differentiation of Hair Follicle Stem Cell Into Keratinocyte, Biomedical Journal (2017)
3. Augmenting Peripheral Nerve Regeneration Using Hair Follicle Stem Cells in Rats, Basic and Clinical Neuroscience (2022)
4. Homing of Allogeneic Nestin-Positive Hair Follicle-Associated Pluripotent Stem Cells After Maternal Transplantation in Experimental Model of Cortical Dysplasia, Biochemistry and Cell Biology (2015)
5. Tissue Engineered Skin Substitutes, Advances in Experimental Medicine and Biology (2018)
6. Hair Follicle Generation by Injections of Adult Human Follicular Epithelial and Dermal Papilla Cells Into Nude Mice, Cell Journal (2017)
7. Promotion of Excisional Wound Repair by a Menstrual Blood-Derived Stem Cell-Seeded Decellularized Human Amniotic Membrane, Biomedical Engineering Letters (2018)
8. Differentiation of Wharton's Jelly Derived Mesenchymal Stem Cells Into Insulin Producing Cells, International Journal of Hematology-Oncology and Stem Cell Research (2018)
Experts (# of related papers)
View all Related Experts
Amirhasan Zarnani (1)
Babak Arjmand (1)