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Collagen-Graft Mixed Cellulose Esters Membrane Maintains Undifferentiated Morphology and Markers of Potential Pluripotency in Feeder-Free Culture of Induced Pluripotent Stem Cells Publisher Pubmed



Lotfalah Moradi S1, 2 ; Hajishafieeha Z3 ; Nojedehi S4 ; Dinarvand V5 ; Hesami Tackallou S6 ; Roy RV7 ; Ardeshirylajimi A4, 8 ; Soleimani M9
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Department of Cell Culture Media, Danesh Azma Cell Company, Tehran, Iran
  3. 3. Department of Microbiology, Qazvin University of Medical Sciences, Qazvin, Iran
  4. 4. Stem Cell Technology Research Center, Tehran, Iran
  5. 5. Department of Physiotherapy, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran
  6. 6. Islamic Azad University, Garmsar Branch, Garmsar, Iran
  7. 7. Center for Research on Environmental Disease and Graduate Center for Toxicology, University of Kentucky, Lexington, KY, United States
  8. 8. Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  9. 9. Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Source: Biologicals Published:2016


Abstract

Induced pluripotent stem cells (iPSCs) are unique and unlimited clinical sources of stem cell therapy for the regenerative medicine. Feeder layer preparation is an important step for iPSCs production, which is expensive, time-consuming and requires conversance. In the present study, we investigated the maintenance of pluripotency, and stemness of the iPSCs through feeder-free culture on a collagen-grafted Mixed Cellulose Esters membrane (MCE-COL) after three passages during twelve days. Results have demonstrated that the iPSCs cultured on MCE-COL membrane had a fine, typical undifferentiated morphology, increased proliferation rate and significant multi-lineage differentiation potential. Alkaline phosphatase (ALP) staining and pluripotency associated gene markers expression further confirmed that iPSCs cultured on the surface of MCE-COL had more ALP positive colonies and enhanced expression of Oct-4, Nanog, Sox-2 and ALP in comparison with MCE and control groups. Since MCE-COL membrane has three dimensional structure and bioactivity, it has the potential for usage in the feeder-free culture of iPSCs, and could be a suitable candidate to use as a feeder layer in stem cells preparation. © 2016 International Alliance for Biological Standardization
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