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In Vitro Osteogenic Differentiation Potential of the Human Induced Pluripotent Stem Cells Augments When Grown on Graphene Oxide-Modified Nanofibers Publisher Pubmed



Saburi E1 ; Islami M2 ; Hosseinzadeh S3 ; Moghadam AS4 ; Mansour RN5 ; Azadian E3 ; Joneidi Z6 ; Nikpoor AR1, 7 ; Ghadiani MH8 ; Khodaii Z2 ; Ardeshirylajimi A3
Authors
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Authors Affiliations
  1. 1. Immunogenetic and Cell Culture Department, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
  2. 2. Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran
  3. 3. Department of Tissue engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Bu-Ali Research Institute, Department of Immunogenetics, Mashhad University of Medical Sciences, Mashhad, Iran
  5. 5. Stem Cell Technology Research Center, Tehran, Iran
  6. 6. Department of Genetics and Molecular Medicine, Zanjan University of Medical Science, Zanjan, Iran
  7. 7. Department of Immunology, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
  8. 8. Department of Nephrology, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Gene Published:2019


Abstract

Due to the several limitations that surgeons are faced during bone tissue implantation there are daily increases in introducing new cell-co-polymer composites for use in bone tissue engineering approaches. In this study tried to develop a suitable nanostructured bio-composite for enhancing osteogenic differentiation of the human induced pluripotent stem cells (iPSCs). Polyvinylidene fluoride-Graphene oxide (PVDF-GO) nanofibers was fabricated by electrospinning and then characterized using scanning electron microscope, tensile and viability assays. After that osteogenic differentiation of the iPSCs was investigated in three groups, including PVDF, PVDF-GO and tissue culture plate as a control group. Alkaline phosphatase activity and calcium content of the iPSCs cultured on PVDF-GO were significantly higher than those cultured on other groups. In addition, Runx2, osteocalcin and osteonectin genes were up regulated in iPSCs cultured on PVDF-GO significantly higher than those cells cultured on PVDF and control. Finally, osteocalcin and osteopontin proteins expression evaluated and the results confirmed higher osteoinductivity of the PVDF-GO nanofibers in comparison with the PVDF nanofibers. According to the results, it was demonstrated that PVDF-GO nanofibers have a great osteoinductive potential and taking together iPSCs-PVDF-GO nanofibrous construct can be an appropriate bio-implant to use for bone tissue engineering applications. © 2019
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