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Poly (3-Hydroxybutyrate-Co-3-Hydroxyvalerate) Improved Osteogenic Differentiation of the Human Induced Pluripotent Stem Cells While Considered As an Artificial Extracellular Matrix Publisher Pubmed



Hosseini FS1 ; Soleimanifar F2 ; Aidun A3, 4 ; Enderami SE5 ; Saburi E6 ; Marzouni HZ7 ; Khani MM8 ; Khojasteh A8 ; Ardeshirylajimi A8
Authors
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Authors Affiliations
  1. 1. Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Dietary supplements and probiotic research center, Alborz University of Medical Sciences, Karaj, Iran
  3. 3. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
  4. 4. Tissues and Biomaterials Research Group (TBRG), Universal Scientific Education and Research Network (USERN), Tehran, Iran
  5. 5. Stem Cell and Regenerative Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering & Biotechnology (NIGEB), Tehran, Iran
  6. 6. Clinical Research Development Center, Imam Hasan Hospital, North Khorasan University of Medical Sciences, Bojnurd, Iran
  7. 7. Department of Immunology, Mashhad University of Medical Sciences, Mashhad, Iran
  8. 8. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Journal of Cellular Physiology Published:2019


Abstract

Cocell polymers can be the best implants for replacing bone defects in patients. The pluripotent stem cells produced from the patient and the nanofibrous polymeric scaffold that can be completely degraded in the body and its produced monomers could be also usable are the best options for this implant. In this study, electrospun poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) nanofibers were fabricated and characterized and then osteogenic differentiation of the human-induced pluripotent stem cells (iPSCs) was investigated while cultured on PHBV scaffold. MTT results showed that cultured iPSCs on PHBV proliferation were increased compared to those cultured on tissue culture polystyrene (TCPS) as the control. Alkaline phosphatase (ALP) activity and calcium content were also significantly increased in iPSCs cultured on PHBV compared to the cultured on TCPS under osteogenic medium. Gene expression evaluation demonstrated that Runx2, collagen type I, ALP, osteonectin, and osteocalcin were upregulated in iPSCs cultured on PHBV scaffold in comparison with those cultured on TCPS for 2 weeks. Western blot analysis have shown that osteocalcin and osteopontin expression as two major osteogenic markers were increased in iPSCs cultured on PHBV scaffold. According to the results, nanofiber-based PHBV has a promising potential to increase osteogenic differentiation of the stem cells and iPSCs-PHBV as a cell-co-polymer construct demonstrated that has a great efficiency for use as a bone tissue engineered bioimplant. © 2018 Wiley Periodicals, Inc.
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