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Vasculo-Osteogenic Keratin-Based Nanofibers Containing Merwinite Nanoparticles and Sildenafil for Bone Tissue Regeneration Publisher Pubmed



Talib Alsudani B1 ; Almusawi MH1 ; Kamil MM2 ; Turki SH3 ; Nasiri Harchegani S4 ; Najafinezhad A4 ; Noory P5 ; Talebi S6 ; Valizadeh H7 ; Sharifianjazi F8 ; Bazli L9 ; Tavakoli M10 ; Mehrjoo M11 ; Firuzeh M10 Show All Authors
Authors
  1. Talib Alsudani B1
  2. Almusawi MH1
  3. Kamil MM2
  4. Turki SH3
  5. Nasiri Harchegani S4
  6. Najafinezhad A4
  7. Noory P5
  8. Talebi S6
  9. Valizadeh H7
  10. Sharifianjazi F8
  11. Bazli L9
  12. Tavakoli M10
  13. Mehrjoo M11
  14. Firuzeh M10
  15. Mirhaj M10
Show Affiliations
Authors Affiliations
  1. 1. Department of Clinical Laboratory Sciences, College of Pharmacy, Mustansiriyah University, Baghdad, Iraq
  2. 2. Department of Pharmaceutics, College of Pharmacy, Mustansiriyah University, Baghdad, Iraq
  3. 3. Department of Plant Biotechnology College of Biotechnology, Al-Nahrain University, Baghdad, Iraq
  4. 4. Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iraq
  5. 5. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iraq
  6. 6. Department of Orthopaedics, Isfahan University of Medical Science, Isfahan, Iraq
  7. 7. Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iraq
  8. 8. Department of Natural Sciences, School of Science and Technology, University of Georgia, Tbilisi, 0171, Georgia
  9. 9. School of Science and Technology, The University of Georgia, Tbilisi, Georgia
  10. 10. Department of Materials Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iraq
  11. 11. Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iraq

Source: International Journal of Pharmaceutics Published:2024


Abstract

Vascularization of bone tissue constructs plays a pivotal role in facilitating nutrient transport and metabolic waste removal during the processes of osteogenesis and bone regeneration in vivo. In this study, a sildenafil (Sil)-loaded nanofibrous scaffold of keratin/Soluplus/merwinite (KS.Me.Sil) was fabricated through electrospinning and the effectiveness of the scaffold was assessed for bone tissue engineering applications. The KS.Me.Sil nanofibrous scaffold exhibited notably enhanced ultimate tensile strength (3.38 vs 2.61 MPa) and elastic modulus (69.83 vs 46.27 MPa) compared to the KS scaffold. The in vitro release of Ca2+, Si4+ and Mg2+ ions and the release of Sil from the nanofibers as well as biodegradability and bioactivity were evaluated for 14 days. Protein adsorption capability and cytocompatibility of the scaffolds were tested. Alkaline phosphatase activity test, Alizarin red staining and qRT-PCR analysis demonstrated that the KS.Me.Sil nanofibers had the best osteogenic activity among other samples. Also, the results of the chorioallantoic membrane assay showed an almost threefold increase in blood vessel density in the group treated with the KS.Me.Sil nanofibers extract compared to the KS. In conclusion, our findings suggest that the electrospun KS.Me.Sil nanofibrous scaffold offers a robust structure with exceptional osteogenic and angiogenic characteristics, making it a promising candidate for bone tissue engineering applications. © 2024 Elsevier B.V.
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