Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Cd171 Multi-Epitope Peptide Design Based on Immuno-Informatics Approach As a Cancer Vaccine Candidate for Glioblastoma Publisher Pubmed



Mohammadzadeh Hosseini Moghri SAH1 ; Mahmoodi Chalbatani G2 ; Ranjbar M1 ; Raposo C3 ; Abbasian A4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Faculty of Biotechnology, Department of Microbial Biotechnology, Amol University of Special Modern Technologies, Amol, Iran
  2. 2. Department of Medical Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Faculdade de Ciencias Farmaceuticas, Universidade Estadual de Campinas (UNICAMP), Campinas, Brazil
  4. 4. Faculty of Basic Sciences, Department of Biology, Semnan University, Semnan, Iran

Source: Journal of Biomolecular Structure and Dynamics Published:2023


Abstract

Glioblastoma (GB) is a common primary malignancy of the central nervous system, and one of the highly lethal brain tumors. GB cells can promote therapeutic resistance and tumor angiogenesis. The CD171 is an adhesion molecule in neuronal cells that is expressed in glioma cells as a regulator of brain development during the embryonic period. CD171 is one of the immunoglobulin-like CAMs (cell adhesion molecules) families that can be associated with prognosis in a variety of human tumors. The multi-epitope peptide vaccines are based on synthetic peptides with a combination of both B-cell epitopes and T-cell epitopes, which can induce specific humoral or cellular immune responses. Moreover, Cholera toxin subunit B (CTB), a novel TLR agonist was utilized in the final construct to polarize CD4+ T cells toward T-helper 1 to induce strong cytotoxic T lymphocytes (CTL) responses. In the present study, several immune-informatics tools were used for analyzing the CD171 sequence and studying the important characteristics of a designed vaccine. The results included molecular docking, molecular dynamics simulation, immune response simulation, prediction and validation of the secondary and tertiary structure, physicochemical properties, solubility, conservancy, toxicity as well as antigenicity and allergenicity of the promising candidate for a vaccine against CD171. The immuno-informatic analyze suggested 12 predicted multi-epitope peptides, whose construction consists of 582 residues long. Therewith, cloning adaptation of the designed vaccine was performed, and eventually sequence was inserted into pET30a (+) vector for the application of the anti-glioblastoma vaccine development. Communicated by Ramaswamy H. Sarma. © 2022 Informa UK Limited, trading as Taylor & Francis Group.
Related Docs
View other Related Docs
1. Computational Design and Analysis of a Poly-Epitope Fusion Protein: A New Vaccine Candidate for Hepatitis and Poliovirus, International Journal of Peptide Research and Therapeutics (2020)
2. Engineering and Design of Promising T-Cell-Based Multi-Epitope Vaccine Candidates Against Leishmaniasis, Scientific Reports (2023)
3. Design of a Multi-Epitope Vaccine Against Sars-Cov-2 Using Immunoinformatics Approach, International Journal of Biological Macromolecules (2020)
4. Computational Design of a Novel Vlp-Based Vaccine for Hepatitis B Virus, Frontiers in Immunology (2020)
5. Immunoinformatics Design of Multivalent Chimeric Vaccine for Modulation of the Immune System in Pseudomonas Aeruginosa Infection, Infection# Genetics and Evolution (2020)
6. Covid-19: A Systematic Review and Update on Prevention, Diagnosis, and Treatment, MedComm (2022)
7. Development of a Multiepitope Vaccine Against Sars-Cov-2: Immunoinformatics Study, JMIR Bioinformatics and Biotechnology (2022)
8. Immunoinformatics Approach to Design a Chimeric Cd70-Peptide Vaccine Against Renal Cell Carcinoma, Journal of Immunology Research (2024)
Experts (# of related papers)
View all Related Experts
Babak Negahdari (3)
Mohammad Reza Pourmand (3)
Other Related Docs
9. Rational Design of B-Cell and T-Cell Multi Epitope-Based Vaccine Against Zika Virus, an in Silico Study, Journal of Biomolecular Structure and Dynamics (2024)
10. Immunoinformatics: In Silico Approaches and Computational Design of a Multi-Epitope, Immunogenic Protein, International Reviews of Immunology (2019)
11. Immunopotentiation of the Engineered Low-Molecular-Weight Pilin Targeting Pseudomonas Aeruginosa: A Combination of Immunoinformatics Investigation and Active Immunization, Molecular Immunology (2020)
12. A Bioinformatics Approach to Introduce Novel Multi-Epitope Vaccines Against Acinetobacter Baumannii Retrieved From Immunogenic Extracellular Loops of Outer Membrane Proteins, Informatics in Medicine Unlocked (2022)
13. Identification of Novel Putative Immunogenic Targets and Construction of a Multi-Epitope Vaccine Against Multidrug-Resistant Corynebacterium Jeikeium Using Reverse Vaccinology Approach, Microbial Pathogenesis (2022)
14. Reverse Vaccinology Approaches to Introduce Promising Immunogenic and Drug Targets Against Antibiotic-Resistant Neisseria Gonorrhoeae: Thinking Outside the Box in Current Prevention and Treatment, Infection# Genetics and Evolution (2023)
15. In Silico Approach for Designing a Novel Recombinant Fusion Protein As a Candidate Vaccine Against Hpv, Current Proteomics (2021)
16. Introduction of Novel Putative Immunogenic Targets Against Proteus Mirabilis Using a Reverse Vaccinology Approach, Infection# Genetics and Evolution (2021)
17. Structure-Based Study of Immune Receptors As Eligible Binding Targets of Coronavirus Sars-Cov-2 Spike Protein, Journal of Molecular Graphics and Modelling (2021)
18. Epitope-Based Immunoinformatics Study of a Novel Hla-Mntc-Sacol0723 Fusion Protein From Staphylococcus Aureus: Induction of Multi-Pattern Immune Responses, Molecular Immunology (2019)
19. In Silico Design and Immunoinformatics Analysis of a Chimeric Vaccine Construct Based on Salmonella Pathogenesis Factors, Microbial Pathogenesis (2023)
20. Subtractive Genomic Analysis for Computational Identification of Putative Immunogenic Targets Against Clinical Enterobacter Cloacae Complex, PLoS ONE (2022)
21. Designing a Chimeric Vaccine Against Colorectal Cancer, International Journal of Cancer Management (2017)
22. Computer-Assisted Multi-Epitopes T-Cell Subunit Covid-19 Vaccine Design, Biomedical and Biotechnology Research Journal (2021)
23. The Effect of in Silico Targeting Pseudomonas Aeruginosa Patatin-Like Protein D, for Immunogenic Administration, Computational Biology and Chemistry (2018)
24. In Silico Designing of Novel Epitope-Based Peptide Vaccines Against Hiv-1, Biotechnology Letters (2024)
25. Inhibition of Sars-Cov-2 Pathogenesis by Potent Peptides Designed by the Mutation of Ace2 Binding Region, Computers in Biology and Medicine (2022)
26. Rational Design of Anti-Angiogenic Peptides to Inhibit Vegf/Vegfr2 Interactions for Cancer Therapeutics, Anti-Cancer Agents in Medicinal Chemistry (2022)
27. In Silico Analysis for Determination and Validation of Iron-Regulated Protein From Escherichia Coli, International Journal of Peptide Research and Therapeutics (2019)
28. Subtractive Genomic Approach Toward Introduction of Novel Immunogenic Targets Against Clostridioides Difficile: Thinking Out of the Box, Microbial Pathogenesis (2022)
29. Defeating a Superbug: A Breakthrough in Vaccine Design Against Multidrug-Resistant Pseudomonas Aeruginosa Using Reverse Vaccinology, PLoS ONE (2023)
30. Investigation of Novel Putative Immunogenic Targets Against Staphylococcus Aureus Using a Reverse Vaccinology Strategy, Infection# Genetics and Evolution (2021)
31. Utilization of Whole Exome Sequencing in Lethal Form of Multiple Pterygium Syndrome: Identification of Mutations in Embryonal Subunit of Acetylcholine Receptor, International Journal of Molecular and Cellular Medicine (2019)
32. In Silico Analyses of Extracellular Proteins of Acinetobacter Baumannii As Immunogenic Candidates, Iranian Journal of Pharmaceutical Research (2022)
33. Vegfr2 Mimicking Peptide Inhibits the Proliferation of Human Umbilical Vein Endothelial Cells (Huvecs) by Blocking Vegf, Anti-Cancer Agents in Medicinal Chemistry (2023)
34. Isolation of a Novel Anti-Kdr3 Single-Chain Variable Fragment Antibody From a Phage Display Library, Iranian Journal of Allergy# Asthma and Immunology (2019)
35. Impacts of the G145r Mutation on the Structure and Immunogenic Activity of the Hepatitis B Surface Antigen: A Computational Analysis, Hepatitis Monthly (2016)
36. Computational Designing of the Ligands of Protein L Affinity Chromatography Based on Molecular Docking and Molecular Dynamics Simulations, Journal of Biomolecular Structure and Dynamics (2023)
37. Genome-Wide Subtraction Analysis and Reverse Vaccinology to Detect Novel Drug Targets and Potential Vaccine Candidates Against Ehrlichia Chaffeensis, Applied Biochemistry and Biotechnology (2023)
38. Construction of a Recombinant B-Cell Epitope Vaccine Based on a Der P1-Derived Hypoallergen: A Bioinformatics Approach, Immunotherapy (2018)
39. In Silico Homology Modeling and Epitope Prediction of Nada As a Potential Vaccine Candidate in Neisseria Meningitidis, International Journal of Molecular and Cellular Medicine (2018)
40. Construction of a Chimeric Flic Including Epitopes of Ompa and Ompk36 As a Multi-Epitope Vaccine Against Klebsiella Pneumonia, Health Biotechnology and Biopharma (2021)
41. An Alphavirus-Derived Self-Amplifying Mrna Encoding Ppsp15-Lmsti1 Fusion Protein for the Design of a Vaccine Against Leishmaniasis, Parasitology International (2022)
42. In Silico Analysis for Determination and Validation of Human Cd20 Antigen 3D Structure, International Journal of Peptide Research and Therapeutics (2019)
43. Designing a Novel Multi-Epitope Dna-Based Vaccine Against Tuberculosis: In Silico Approach, Jundishapur Journal of Microbiology (2017)
44. Library-Based Lead Compound Discovery for Cs-1 Protein in Multiple Myeloma: Homology Modelling, Molecular Dynamic Simulations, Virtual Screening and Molecular Docking, Molecular Simulation (2021)
45. Investigation of Biological Activities of Two Cultivars of Cicer Arietinum Proteins Mass Associated With Alzheimer's Disease, Proteins: Structure# Function and Bioinformatics (2023)
46. Efficient Detection of Eukaryotic Calcium-Sensing Receptor (Casr) by Polyclonal Antibody Against Prokaryotic Expressed Truncated Casr, Molecular Biology Reports (2020)
47. Deletion of Loop Fragment Adjacent to Active Site Diminishes the Stability and Activity of Exo-Inulinase, International Journal of Biological Macromolecules (2016)
48. Human Il-2Rα Subunit Binding Modulation of Il-2 Through a Decline in Electrostatic Interactions: A Computational and Experimental Approach, PLoS ONE (2022)
49. A Systematic Review on Popularity, Application and Characteristics of Protein Secondary Structure Prediction Tools, Current Drug Discovery Technologies (2019)
50. Rational Design of Dkk3 Structure-Based Small Peptides As Antagonists of Wnt Signaling Pathway and in Silico Evaluation of Their Efficiency, PLoS ONE (2017)