Designing a Chimeric Vaccine Against Colorectal Cancer Publisher



Malekshahi ZV¹ ; Rajabibazl M^{2, 3} ; Ebrahimizadeh W⁴ ; Amani J⁵ ; Negahdari B¹
Source: International Journal of Cancer Management Published:2017

Abstract

Background: Colorectal cancer is the major cause of death worldwide. Recently, cancer immunotherapy has been used in cancer prevention and treatment which has led to immune system response to cancerous cells and their elimination. Recent studies were conducted to specifically target tumor marker to design vaccine. Objectives: Here, we designed a vaccine chimeric including CEA and CA19-9 against colorectal cancer. Methods: The construct was analyzed, using bioinformatics tools and servers. The physicochemical properties, structures, anti-genicity, stability, MHC binding properties, and ligand-receptor docking of chimeric protein (CE-CA) were predicted. Results: The results showed the structure of CE-CA has suitable form and separates each domain; furthermore, the epitope mapping did not change after combination. Conclusions: The results showed that the construct can be appropriate vaccine against colorectal cancer and could generate potent immune response against this cancer. In silico tools are useful to design vaccine. © 2017, Cancer Research Center (CRC), Shahid Beheshti University of Medical Sciences.