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Construction of a Chimeric Flic Including Epitopes of Ompa and Ompk36 As a Multi-Epitope Vaccine Against Klebsiella Pneumonia Publisher



Goodarzi NN1 ; Fereshteh S2 ; Sabzi S3 ; Shahbazi S3 ; Badmasti F2, 4
Authors
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Authors Affiliations
  1. 1. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran
  3. 3. Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
  4. 4. Microbiology research center (MRC), Pasteur Institute of Iran, Tehran, Iran

Source: Health Biotechnology and Biopharma Published:2021


Abstract

OmpA and OmpK36 from K. pneumoniae as the antigens and FliC from Salmonella typhimurium as the adjuvant were applied to construct a multi-epitope vaccine. B-cell, T-cell, and IL-17-epitopes were identified and a construct was modeled. Molecular docking was performed to assess the interaction between chimeric FliC and TLR-5. Downstream analysis including antigenicity, allergenicity, IFN-γ and IL-4 epitopes prediction was done. This construction covers both B-cell and T-cell epitopes as well as IFN-γ and IL-4 epitopes, but no IL-17 epitope was detected. We used two other known epitopes (IEDB epitope ID 43662 and epitope ID 57417) that induce the IL-17 release. According to the result, the multi-epitope protein is probable antigen and not an allergen. This construction was stable, hydrophilic, and has no transmembrane helixes. The computer-aided analysis imply that this protein is an acceptable candidate for immunization against K. pneumoniae. © 2021 Health Biotechnology And Biopharma. All right reserved.
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