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Computational Design of a Novel Vlp-Based Vaccine for Hepatitis B Virus Publisher Pubmed



Mobini S1 ; Chizari M2 ; Mafakher L3 ; Rismani E4 ; Rismani E4
Authors
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Authors Affiliations
  1. 1. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Medical Biotechnology, School of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Medicinal Plant Research Center, Ahvaz Jundishapur of Medical Science, Ahvaz, Iran
  4. 4. Department of Biology, Payam Noor University, Tehran, Iran
  5. 5. Molecular Medicine Department, Pasteur Institute of Iran, Tehran, Iran

Source: Frontiers in Immunology Published:2020


Abstract

Hepatitis B virus (HBV) is a global virus responsible for a universal disease burden for millions of people. Various vaccination strategies have been developed using viral vector, nucleic acid, protein, peptide, and virus-like particles (VLPs) to stimulate favorable immune responses against HBV. Given the pivotal role of specific immune responses of hepatitis B surface antigen (HBsAg) and hepatitis B core antigen (HBcAg) in infection control, we designed a VLP-based vaccine by placing the antibody-binding fragments of HBsAg in the major immunodominant region (MIR) epitope of HBcAg to stimulate multilateral immunity. A computational approach was employed to predict and evaluate the conservation, antigenicity, allergenicity, and immunogenicity of the construct. Modeling and molecular dynamics (MD) demonstrated the folding stability of HBcAg as a carrier in inserting Myrcludex and “a” determinant of HBsAg. Regions 1–50 and 118–150 of HBsAg were considered to have the highest stability to be involved in the designed vaccine. Molecular docking revealed appropriate interactions between the B cell epitope of the designed vaccine and the antibodies. Totally, the final construct was promising for inducing humoral and cellular responses against HBV. © Copyright © 2020 Mobini, Chizari, Mafakher, Rismani and Rismani.
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