Ros-Mediated Heme Degradation and Cytotoxicity Induced by Iron Nanoparticles: Hemoglobin and Lymphocyte Cells As Targets

Ros-Mediated Heme Degradation and Cytotoxicity Induced by Iron Nanoparticles: Hemoglobin and Lymphocyte Cells As Targets Publisher Pubmed

Mirzaei S¹ ; Hadadi Z¹ ; Attar F² ; Mousavi SE³ ; Zargar SS⁴ ; Tajik A⁴ ; Saboury AA⁵ ; Rezayat SM^{3, 6} ; Falahati M⁷

Show Affiliations

1. Department of Cellular and Molecular Biology, Faculty of Advance Science and Technology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran
2. Department of Biology, Faculty of Food Industry & Agriculture, Standard Research Institute (SRI), Karaj, Iran
3. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Toxicologyâ€“Pharmacology, Faculty of Pharmacy, Pharmaceutical Science Branch, Islamic Azad University (IAUPS), Tehran, Iran
5. Department of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, Iran
6. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
7. Department of Nanotechnology, Faculty of Advance Science and Technology, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran

Source: Journal of Biomolecular Structure and Dynamics Published:2018

Abstract

Nanoparticles (NPs) due to their small size and high surface area induce remarkable adverse effects on the biological systems. However, the exact mechanism by which NPs interacted with biological system and induce their adverse effects is still an enigma. Herein, the interaction of zero valent iron NPs (ZVFe NPs) with human hemoglobin (Hb) was evaluated using a variety of techniques including circular dichroism, fluorescence, and UV–visible (UV–vis) spectroscopy methods. Also, the cytotoxicity of ZVFe NPs on the human lymphocyte cell line as a model of blood system cell line was investigated by reactive oxygen species (ROS), caspase-9, and caspase-3 activities assays. It was revealed that ZVFe NP interaction resulted in heme displacement and degradation and induction of protein cabonylation. It was also shown that ZVFe NPs impaired the complexity of lymphocyte cells through ROS generation and apoptotic pathway. Together, these data suggest that NPs influence the biological system and induce adverse effects through ROS generation. © 2017, © 2017 Informa UK Limited, trading as Taylor & Francis Group.

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Style	Citing Format
MLA	Mirzaei S, et al.. "Ros-Mediated Heme Degradation and Cytotoxicity Induced by Iron Nanoparticles: Hemoglobin and Lymphocyte Cells As Targets." Journal of Biomolecular Structure and Dynamics, vol. 36, no. 16, 2018, pp. 4235-4245.
APA	Mirzaei S, Hadadi Z, Attar F, Mousavi SE, Zargar SS, Tajik A, Saboury AA, Rezayat SM, Falahati M (2018). Ros-Mediated Heme Degradation and Cytotoxicity Induced by Iron Nanoparticles: Hemoglobin and Lymphocyte Cells As Targets. Journal of Biomolecular Structure and Dynamics, 36(16), 4235-4245.
Chicago	Mirzaei S, Hadadi Z, Attar F, Mousavi SE, Zargar SS, Tajik A, Saboury AA, Rezayat SM, Falahati M. "Ros-Mediated Heme Degradation and Cytotoxicity Induced by Iron Nanoparticles: Hemoglobin and Lymphocyte Cells As Targets." Journal of Biomolecular Structure and Dynamics 36, no. 16 (2018): 4235-4245.
Harvard	Mirzaei S et al. (2018) 'Ros-Mediated Heme Degradation and Cytotoxicity Induced by Iron Nanoparticles: Hemoglobin and Lymphocyte Cells As Targets', Journal of Biomolecular Structure and Dynamics, 36(16), pp. 4235-4245.
Vancouver	Mirzaei S, Hadadi Z, Attar F, Mousavi SE, Zargar SS, Tajik A, et al.. Ros-Mediated Heme Degradation and Cytotoxicity Induced by Iron Nanoparticles: Hemoglobin and Lymphocyte Cells As Targets. Journal of Biomolecular Structure and Dynamics. 2018;36(16):4235-4245.
BibTex	@article{ author = {Mirzaei S and Hadadi Z and Attar F and Mousavi SE and Zargar SS and Tajik A and Saboury AA and Rezayat SM and Falahati M}, title = {Ros-Mediated Heme Degradation and Cytotoxicity Induced by Iron Nanoparticles: Hemoglobin and Lymphocyte Cells As Targets}, journal = {Journal of Biomolecular Structure and Dynamics}, volume = {36}, number = {16}, pages = {4235-4245}, year = {2018} }
RIS	TY - JOUR AU - Mirzaei S AU - Hadadi Z AU - Attar F AU - Mousavi SE AU - Zargar SS AU - Tajik A AU - Saboury AA AU - Rezayat SM AU - Falahati M TI - Ros-Mediated Heme Degradation and Cytotoxicity Induced by Iron Nanoparticles: Hemoglobin and Lymphocyte Cells As Targets JO - Journal of Biomolecular Structure and Dynamics VL - 36 IS - 16 SP - 4235 EP - 4245 PY - 2018 ER -

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