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Cardiac Failure Detection in 30 Minutes: New Approach Based on Gold Nanoparticles Publisher Pubmed



Namdari M1 ; Negahdari B2 ; Cheraghi M1 ; Aiyelabegan HT3 ; Eatmadi A2, 4
Authors
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Authors Affiliations
  1. 1. Department of Cardiology, Lorestan University of Medical Sciences, Khorramabad, Iran
  2. 2. Department of Medical Biotechnology, School of Advance Science in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Medical Biotechnology, School of Advance Science in Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran

Source: Journal of Microencapsulation Published:2017


Abstract

Cardiac failure occurs when heart is unable to pump sufficiently to maintain blood flow to meet the body’s needs. The aim of this work is to detect highly expressed genes: follistatin-related protein 1 (FSTL1) in heart failure within 30 minutes, using gold nanoparticles. Gold nanoparticles were prepared by citrate reduction of HAuCl4 3H2O; probe sequence was designed based on the FSTL1 gene region. Preparation of gold nanoprobes (AuNPs) proceeded by treating all the containers with DEPC-treated water, followed by reduction and conjugation. Transmission electron microscopy shows that AuNPs were 10–15 nm in size. The concentration of the nanoprobes was 2.1 nM, and they bind to target. Real-time PCR shows an over-expression of FSTL1 and FSTL3 in heart failure (p <.05). Our data showed that elevated expression of the FSTL1 and FSTL3 is a marker of heart failure as detected within 30 minutes by the synthesised AuNPs; the method is accurate and fast. © 2017 Informa UK Limited, trading as Taylor & Francis Group.