Immobilization of Cellulase Enzyme Onto Magnetic Nanoparticles: Applications and Recent Advances Publisher



Khoshnevisan K^{1, 2} ; Vakhshiteh F³ ; Barkhi M⁴ ; Baharifar H⁵ ; Poorakbar E⁶ ; Zari N⁷ ; Stamatis H⁸ ; Bordbar AK⁹ Show Affiliations
Source: Molecular Catalysis Published:2017

Abstract

Among the industrial enzymes, cellulase plays a central role in biotechnology and agricultural applications such as foods, chemicals, detergents, cosmetics, pulp, and paper. Most of these applications suffer from low thermal and storage stability of cellulase, contamination of products by enzyme and lake of enzyme reusability that make process inappropriate in economic viewpoint. For overcoming these issues and improve the stability and reusability of the enzyme, the immobilization methods could employ. Recently, the immobilization of cellulase on nanomaterials is under more considerable attention due to the significant surface properties of these supports. However, the application of these supports is also restricted by troubles in recovery, e.g., via centrifugation or filtration. This restriction could be solved using magnetic nanoparticles (MNPs) that can simply separate from the reaction media by using a simple magnet. MNPs have been commonly used for enzyme immobilization in recent years due to this practical advantage and their low toxicity. In this review, we attempt to provide a more detailed overview of the applications and recent advances of immobilization of cellulase enzyme onto different functionalized MNPs. At last, we tried to present the future perspective of this research area such as the enzyme reusability, the facile recovery way of cellulase, and the efficient biotechnology production process. © 2017 Elsevier B.V.