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Efficient Osteogenic Differentiation of the Dental Pulp Stem Cells on Β-Glycerophosphate Loaded Polycaprolactone/Polyethylene Oxide Blend Nanofibers Publisher Pubmed



Hosseini FS1 ; Enderami SE2 ; Hadian A1 ; Abazari MF3 ; Ardeshirylajimi A4 ; Saburi E5 ; Soleimanifar F6 ; Nazemisalman B7
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Authors Affiliations
  1. 1. Stem Cell Technology Research Center, Tehran, Iran
  2. 2. Stem Cell and Regenerative Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering & Biotechnology (NIGEB), Tehran, Iran
  3. 3. Department of Genetics, Tehran Medical Science Branch, Islamic Azad University, Tehran, Iran
  4. 4. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Clinical Research Development Center, Imam Hasan Hospital, North Khorasan University of Medical Sciences, Bojnurd, Iran
  6. 6. Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran
  7. 7. Department of Pediatrics, Faculty of Dental, Zanjan University of Medical Sciences, Zanjan, Iran

Source: Journal of Cellular Physiology Published:2019


Abstract

Hard tissue lesion treatment in oral and maxillofacial has been challenging because of tissue complexities. This study aimed to investigate novel biopolymeric construct effects on the osteogenic differentiation potential of the dental pulp stem cells (DPSCs) for introducing a cell copolymer bioimplant. A blended polycaprolactone (PCL)-polyethylene oxide (PEO) was fabricated using electrospinning, simultaneously filled by β-glycerophosphate (β-GP). After that biocompatibility and release kinetics of the PCL-PEO+β-GP was evaluated and compared with PCL-PEO and then the osteogenic differentiation potential of the DPSCs was examined while being cultured on the scaffolds and compared with those cultured on the culture plate. The results demonstrated that scaffolds have not any cytotoxicity and β-GP can release in a long-term manner. Alkaline phosphatase activity and calcium content were significantly increased in DPSCs while being cultured on the PCL-PEO+β-GP compared with the other groups. Runt-related transcription factor 2, collagen type-I, osteonectin, and osteocalcin (OSC) genes expression was upregulated in DPSCs cultured on the PCL-PEO+β-GP and was significantly higher than those cultured on the PCL-PEO. Immunocytochemistry result also confirmed the positive effects of PCL-PEO+β-GP on the osteogenic differentiation of the DPSCs by presenting a higher OSC protein expression. According to the results, incorporation of the β-GP in PCL-PEO makes a better construct for osteogenic induction into the stem cells and it could be also considered as a great promising candidate for bone, oral, and maxillofacial tissue engineering applications. © 2019 Wiley Periodicals, Inc.
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