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Promoted Osteogenic Differentiation of Human Induced Pluripotent Stem Cells Using Composited Polycaprolactone/Polyvinyl Alcohol/Carbopol Nanofibrous Scaffold Publisher



Abazari MF1 ; Torabinejad S2 ; Zare Karizi S3 ; Enderami SE4 ; Samadian H5 ; Hajatibirgani N6 ; Norouzi S6 ; Nejati F7 ; Al Bahash A8 ; Mansouri V9
Authors
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Authors Affiliations
  1. 1. Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Clinical Medicine and Surgery, University of Naples Federico II, Naples, Italy
  3. 3. Department of Biology, Varamin Pishva Branch, Islamic Azad University, Pishva, Varamin, Iran
  4. 4. Department of Stem Cell and Regenerative Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
  5. 5. Dental Implants Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
  6. 6. Department of Biology, Faculty of Science and Research, Islamic Azad University, Tehran, Iran
  7. 7. Institute of Molecular Biology, Vrije Universiteit Brussel, Brussels, Belgium
  8. 8. School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  9. 9. Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Journal of Drug Delivery Science and Technology Published:2022


Abstract

Scaffold as a major part of tissue engineering plays an important role in the repairing process of the bone lesions. The scaffold's hydrophilicity plays a fundamental role in the initial cell attachment, and their growth, proliferation and differentiation processes. The aim of this study was to investigate the impact of hydrophilicity increasing in the hydrophobic scaffolds while combined with hydrophilic polymers on the osteogenic differentiation of induced pluripotent stem cells (iPSCs). In the present study, hydrophilicity of Polycaprolactone (PCL) electrospun nanofibers was improved using incorporating polyvinyl alcohol (PVA) and Carbopol (CP). Water uptake assay was confirmed that the hydration value of the PCL/PVA/CP was significantly increased in comparison with PCL and PCL/PVA nanofibers. In addition, the viability of the iPSCs grown on the PCL/PVA/CP was also significantly increased in comparison with the other two groups. Osteogenic differentiation evaluation demonstrated that the highest ALP activity and total calcium was detected in the iPSCs grown on the PCL/PVA/CP compared to the cells cultured on the PCL/PVA nanofibers. Although these values in the iPSCs cultured on the PCL/PVA was also significantly higher than those cells cultured on the PCL as a control group. Bone-related gene expression was also examined in the iPSCs cultured on the fabricated scaffolds. The results revealed that the highest expression level of genes was detected in the cells grown on the PCL/PVA/CP nanofibers. Taken together, these results indicated that the PCL/PVA/CP nanofibrous scaffold has greater osteoinductive potential than PCL/PVA and PCL nanofibers and could be considered as a potential construct in bone tissue engineering applications. © 2022 Elsevier B.V.
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