Multifunctional Hybrid Magnetic Microgel Synthesis for Immune-Based Isolation and Post-Isolation Culture of Tumor Cells

Multifunctional Hybrid Magnetic Microgel Synthesis for Immune-Based Isolation and Post-Isolation Culture of Tumor Cells Publisher Pubmed

Seyfoori A^{1, 2, 5} ; Seyyed Ebrahimi SA^{1, 3, 4} ; Samiei E^{2, 3, 4} ; Akbari M⁵

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1. Advanced Magnetic Materials Research Center, College of Engineering, University of Tehran, Tehran, 14399-57131, Iran
2. Laboratory for Innovations in Micro Engineering (LiME), Department of Mechanical Engineering, University of Victoria, Victoria, V8P 5C2, Canada
3. Center for Biomedical Research, University of Victoria, Victoria, V8P 5C2, Canada
4. Center for Advanced Materials and Related Technologies, University of Victoria, Victoria, V8P 5C2, Canada
5. Biomaterials and Tissue Engineering Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, 1665659911, Iran

Source: ACS Applied Materials and Interfaces Published:2019

Abstract

Circulating tumor cells are of utmost importance among various biomarkers in liquid biopsies as a prognosis indicator of metastasis as well as in chemotherapeutic monitoring. This study introduces an efficient tool composed of soft nano/hybrid immune microgels for magnetic isolation of targeted tumor cells. The development process involves the in situ synthesis of magnetic nanoparticles within the three-dimensional matrix of thermoresponsive microgels. Surface modification and anti-EpCAM conjugation are adjusted by changing the temperature, and a conjugation efficiency of around 70% is achieved by using a protein G linker. Anti-EpCAM-conjugated nano/hybrid magnetic microgels are used to isolate EpCAM-expressing breast adenocarcinoma MCF-7 cells from culture media and whole blood with an efficiency of 75 and 70%, respectively. Furthermore, we demonstrate the ability of the hybrid microgels to isolate cancer cells with a purity of 65% and culture the cells post-isolation for further drug studies. The multifunctional hybrid microcarriers reported in this work can be potentially used for continuous monitoring of cancers and in personalized medicine. © 2019 American Chemical Society.

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Hamid Akbari Javar (2)

Seyed Mohammad Kazem Aghamir Mohammad Ali (2)

Style	Citing Format
MLA	Seyfoori A, et al.. "Multifunctional Hybrid Magnetic Microgel Synthesis for Immune-Based Isolation and Post-Isolation Culture of Tumor Cells." ACS Applied Materials and Interfaces, vol. 11, no. 28, 2019, pp. 24945-24958.
APA	Seyfoori A, Seyyed Ebrahimi SA, Samiei E, Akbari M (2019). Multifunctional Hybrid Magnetic Microgel Synthesis for Immune-Based Isolation and Post-Isolation Culture of Tumor Cells. ACS Applied Materials and Interfaces, 11(28), 24945-24958.
Chicago	Seyfoori A, Seyyed Ebrahimi SA, Samiei E, Akbari M. "Multifunctional Hybrid Magnetic Microgel Synthesis for Immune-Based Isolation and Post-Isolation Culture of Tumor Cells." ACS Applied Materials and Interfaces 11, no. 28 (2019): 24945-24958.
Harvard	Seyfoori A et al. (2019) 'Multifunctional Hybrid Magnetic Microgel Synthesis for Immune-Based Isolation and Post-Isolation Culture of Tumor Cells', ACS Applied Materials and Interfaces, 11(28), pp. 24945-24958.
Vancouver	Seyfoori A, Seyyed Ebrahimi SA, Samiei E, Akbari M. Multifunctional Hybrid Magnetic Microgel Synthesis for Immune-Based Isolation and Post-Isolation Culture of Tumor Cells. ACS Applied Materials and Interfaces. 2019;11(28):24945-24958.
BibTex	@article{ author = {Seyfoori A and Seyyed Ebrahimi SA and Samiei E and Akbari M}, title = {Multifunctional Hybrid Magnetic Microgel Synthesis for Immune-Based Isolation and Post-Isolation Culture of Tumor Cells}, journal = {ACS Applied Materials and Interfaces}, volume = {11}, number = {28}, pages = {24945-24958}, year = {2019} }
RIS	TY - JOUR AU - Seyfoori A AU - Seyyed Ebrahimi SA AU - Samiei E AU - Akbari M TI - Multifunctional Hybrid Magnetic Microgel Synthesis for Immune-Based Isolation and Post-Isolation Culture of Tumor Cells JO - ACS Applied Materials and Interfaces VL - 11 IS - 28 SP - 24945 EP - 24958 PY - 2019 ER -

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