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In Vitro Elimination of El4 Cancer Cells From Spermatogonia Stem Cells by Mirna-143- a Nd 206-Loaded Folic Acid-Conjugated Plga Nanoparticles Publisher Pubmed



Shams A1, 2 ; Shabani R1, 2 ; Asgari H2 ; Karimi M3 ; Najafi M4 ; Asgharijafarabadi M5, 6 ; Razavi SM7 ; Miri SR8 ; Abbasi M9 ; Mohammadi A2 ; Koruji M1, 2
Authors
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Authors Affiliations
  1. 1. Stem Cell and Regenerative Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Statistics and Epidemiology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
  6. 6. Road Traffic Injury Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  7. 7. Clinic of Hematology and Oncology, Firoozgar Hospital, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  8. 8. Department of Surgical Oncology, Cancer Institute, Tehran University of Medical Science, Tehran, Iran
  9. 9. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Nanomedicine Published:2022


Abstract

Aim: MiRNA's-143 and-206 are powerful apoptotic regulators in cancer cells. This study aimed to use miRNA-143- A nd 206-loaded poly(lactic-co-glycolic) acid (PLGA) nanoparticles conjugated with folic acid to induce apoptosis in the EL4 cancer cells. Materials & methods: The therapy was conducted in six groups: Treatment with both miRNAs simultaneously (mixed miRNAs), miRNA-206 treatment, miRNA-143 treatment, blank PLGA, blank polyethylenimine (PEI) and complex PEI-miRNAs. Results: In terms of viability, in mixed miRNAs no synergistic effect was observed on EL4 cell elimination. However, in the single miRNA-206 group, a stronger apoptotic effect was observed than the mixed miRNAs group and single miRNA-143 group alone. Conclusion: MiRNAs' apoptotic induction effects in cancer cells were found to be remarkable. © 2022 Future Medicine Ltd.
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