Acute Cytotoxic Effects of Titanium Dioxide (Tio2) and Chronic Exposure to Safe Dose of Nanoparticle on Hepatocarcinoma Cell Line (Hepg2) Publisher



Shajari Z¹ ; Golestani A¹ ; Nowrouzi A¹ Show Affiliations
Source: Nanomedicine Research Journal Published:2021

Abstract

Objective(s): To address cellular response to nanosized particles, we designed in vitro cytotoxic effects of titanium dioxide nanoparticle was assessed on hepatocarcinoma cell line (HepG2) with novel UVA exposure. Methods: Cellular morphology, cell viability, and membrane leakage of lactate dehydrogenase were used to evaluate the acute cytotoxic effect of TiO2 after 24 hours of exposure. To determine the chronic exposure effects, Hepatocarcinoma cell lines were treated with 125 and 250ppm of TiO2 in 4 consecutive passages lasting 25days. Results: Obvious changes in cellular morphology like cell shrinkage and rounded appearance and cytoplasm granulation was observed at 2500ppm and higher concentration.Cell count decreased during four passages, while cellular oxidative responses such as nitric oxide production, and membrane lipid peroxidation, and total cell protein showed significant increases compared with controls. Conclusions: These results suggest that chronic exposure even to the safe doses of nanosized particles can stimulate cellular oxidative and inflammatory responses. Copyright© 2021, the Authors | Publishing Rights, ASPI.