Two Triacylglycerol Pathway Genes, Ctdnep1 and Lpin1, Are Down-Regulated by Hsa-Mir-122-5P in Hepatocytes

Two Triacylglycerol Pathway Genes, Ctdnep1 and Lpin1, Are Down-Regulated by Hsa-Mir-122-5P in Hepatocytes Pubmed

Naderi M¹ ; Pazouki A² ; Arefian E³ ; Hashemi SM⁴ ; Jamshidiadegani F⁵ ; Gholamalamdari O⁵ ; Soudi S⁶ ; Azadmanesh K⁷ ; Samiee SM⁸ ; Merat S¹ ; Fesharaki MG⁹ ; Mondanizadeh M¹⁰ ; Vasei M¹¹ ; Soleimani M¹²

Show Affiliations

1. Liver and pancreatobiliary diseases research center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Tehran, Iran
2. Minimally Invasive Surgery Research Center, Iran University of Medical Sciences, Tehran, Iran
3. Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, 1417614411, Iran
4. Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5. Stem Cell Technology Research Center, Tehran, Iran
6. Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
7. Department of Virology, Pasteur Institute of Iran, Tehran, Iran
8. Food and Drug Laboratory Research Center, Tehran, Iran
9. Department of Biostatistics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
10. Department of Biotechnology and Molecular Medicine, Arak University of Medical Sciences, Arak, Iran
11. Department of Pathology, Tehran University of Medical Sciences, Shariati Hospital, Tehran, Iran
12. Department of Hematology, Tarbiat Modares University, Tehran, Iran

Source: Archives of Iranian Medicine Published:2017

Abstract

Background: Expression of miR-122 is highly specific to hepatocytes of the liver. This miRNA is involved in lipid hemostasisof the tissue; however, there is no comprehensive understanding of its function in lipid hemostasis. Materials and Methods: Since hepatocytes are responsible for part of Triacylglycerol (TAG) synthesis in the body, we hypothesized that miR-122, as the most abundant miRNA in the tissue, might regulate TAG metabolism by targeting key enzymes that are involved in its production pathway. A systematic computational analysis of putative targets of miR-122 identified CTDNEP1 and LPIN1 genes in the TAG pathway. We used dual-luciferase reporter assay, quantitative RT-PCR as well as western blot to confirm the repressive effect of miR-122 on CTDNEP1 and LPIN1 in TAG pathway. Results: Real time PCR on liver needle biopsies with hepatosteatosis showed that miR-122 is up-regulated in hepatosteatosis. Surprisingly, the protein and RNA level of identified targets of miR-122 are also up-regulated in clinical samples, probably as a disproportionate feedback response to the high level of miR-122. Conclusion: Our findings suggest that up-regulation of miR-122 can trigger the compensatory response of LPIN1 and CTDNEP1 in hepatosteatosis. © 2017, Academy of Medical Sciences of I.R. Iran. All rights reserved.

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Style	Citing Format
MLA	Naderi M, et al.. "Two Triacylglycerol Pathway Genes, Ctdnep1 and Lpin1, Are Down-Regulated by Hsa-Mir-122-5P in Hepatocytes." Archives of Iranian Medicine, vol. 20, no. 3, 2017, pp. 165-171.
APA	Naderi M, Pazouki A, Arefian E, Hashemi SM, Jamshidiadegani F, Gholamalamdari O, Soudi S, Azadmanesh K, Samiee SM, Merat S, Fesharaki MG, Mondanizadeh M, Vasei M, Soleimani M (2017). Two Triacylglycerol Pathway Genes, Ctdnep1 and Lpin1, Are Down-Regulated by Hsa-Mir-122-5P in Hepatocytes. Archives of Iranian Medicine, 20(3), 165-171.
Chicago	Naderi M, Pazouki A, Arefian E, Hashemi SM, Jamshidiadegani F, Gholamalamdari O, Soudi S, et al.. "Two Triacylglycerol Pathway Genes, Ctdnep1 and Lpin1, Are Down-Regulated by Hsa-Mir-122-5P in Hepatocytes." Archives of Iranian Medicine 20, no. 3 (2017): 165-171.
Harvard	Naderi M et al. (2017) 'Two Triacylglycerol Pathway Genes, Ctdnep1 and Lpin1, Are Down-Regulated by Hsa-Mir-122-5P in Hepatocytes', Archives of Iranian Medicine, 20(3), pp. 165-171.
Vancouver	Naderi M, Pazouki A, Arefian E, Hashemi SM, Jamshidiadegani F, Gholamalamdari O, et al.. Two Triacylglycerol Pathway Genes, Ctdnep1 and Lpin1, Are Down-Regulated by Hsa-Mir-122-5P in Hepatocytes. Archives of Iranian Medicine. 2017;20(3):165-171.
BibTex	@article{ author = {Naderi M and Pazouki A and Arefian E and Hashemi SM and Jamshidiadegani F and Gholamalamdari O and Soudi S and Azadmanesh K and Samiee SM and Merat S and Fesharaki MG and Mondanizadeh M and Vasei M and Soleimani M}, title = {Two Triacylglycerol Pathway Genes, Ctdnep1 and Lpin1, Are Down-Regulated by Hsa-Mir-122-5P in Hepatocytes}, journal = {Archives of Iranian Medicine}, volume = {20}, number = {3}, pages = {165-171}, year = {2017} }
RIS	TY - JOUR AU - Naderi M AU - Pazouki A AU - Arefian E AU - Hashemi SM AU - Jamshidiadegani F AU - Gholamalamdari O AU - Soudi S AU - Azadmanesh K AU - Samiee SM AU - Merat S AU - Fesharaki MG AU - Mondanizadeh M AU - Vasei M AU - Soleimani M TI - Two Triacylglycerol Pathway Genes, Ctdnep1 and Lpin1, Are Down-Regulated by Hsa-Mir-122-5P in Hepatocytes JO - Archives of Iranian Medicine VL - 20 IS - 3 SP - 165 EP - 171 PY - 2017 ER -

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