Oxali-Palladium Nanoparticle Synthesis, Characterization, Protein Binding, and Apoptosis Induction in Colorectal Cancer Cells Publisher Pubmed



Golestannezhad N¹ ; Divsalar A¹ ; Badalkhanikhamseh F² ; Rasouli M^{3, 4} ; Seyedarabi A⁵ ; Ghalandari B⁶ ; Ding X⁶ ; Goli F¹ ; Bekeschus S⁷ ; Movahedi AAM⁵ ; Moghadam ME⁸ Show Affiliations
Source: Journal of Materials Science: Materials in Medicine Published:2024

Abstract

This paper focuses on the synthesis of nano-oxali-palladium coated with turmeric extract (PdNPs) using a green chemistry technique based on the reduction in the Pd (II) complex by phytochemicals inherent in turmeric extract. PdNPs were examined and characterized using Field Emission Scanning Electron Microscopy (FESEM), Dynamic Light Scattering (DLS), Fourier Transform Infrared (FTIR), and Atomic Force Microscopy (AFM). Using different spectroscopic and molecular dynamics simulations, a protein-binding analysis of the produced nanoparticle was conducted by observing its interaction with human serum albumin (HSA). Lastly, the cytotoxic effects and apoptotic processes of PdNPs were studied against the HCT116 human colorectal cell line using the MTT assay and flow cytometry tests. According to the findings, PdNPs with spherical and homogenous morphology and a size smaller than 100 nm were generated. In addition, they can induce apoptosis in colorectal cancer cells in a dose-dependent manner with a lower Cc50 (78 µL) than cisplatin and free oxali-palladium against HCT116 cells. The thermodynamic characteristics of protein binding of nanoparticles with HSA demonstrated that PdNPs had a great capacity for quenching and interacting with HSA through hydrophobic forces. In addition, molecular dynamics simulations revealed that free oxali-palladium and PdNP attach to the same area of HSA via non-covalent interactions. It is conceivable to indicate that the synthesized PdNPs are a potential candidate for the construction of novel, nature-based anticancer treatments with fewer side effects and a high level of eco-friendliness. Graphical Abstract: [Figure not available: see fulltext.] © 2023, The Author(s).