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Blockage of Hotair Reduced Cell Proliferation in Human Ovarian Cancer Cells Through Upregulation of Akt2 Publisher



Sabet M1 ; Sharifi M2 ; Heidari M3 ; Kazemi M2 ; Babaei N1
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Authors Affiliations
  1. 1. Department of Cell Biology and Genetics, Bushehr Branch, Islamic Azad University, Bushehr, Iran
  2. 2. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 81744-176, Iran
  3. 3. Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran

Source: Indian Journal of Gynecologic Oncology Published:2019


Abstract

Purpose: Ovarian cancer (OC) is the most leading cause of cancer-related death in women. lncRNA HOTAIR is emerging as an onco-lncRNA that its overexpression provokes progression and metastasis in OC. In this study, we focused on evaluating the efficiency of antisense-based HOTAIR inhibition in OC tumor cells death. Methods: We transfected antisense LNA GapmeR into OC cell line (SKOV3). Then, the survival rates of SKOV3 were measured by MTT at all three time points after transfection. Moreover, we examined the expression level of AKT2 in SKOV3 under the influence of GapmeRs using qPCR at 24, 48 and 72 h after transfection. Results: The viability of SKOV3 was significantly reduced over time under the suppression of lncRNA HOTAIR. The qPCR results disclosed the remarkable increase in AKT2 expression level via inhibition of HOTAIR in SKOV3 cell line. Conclusion: Our data imply that inhibition of HOTAIR by antisense LNA GapmeR induced OC cell death. Hence, we concluded that antisense-based strategies might be considered as a potential therapeutic approach in OC treatment. © 2019, Association of Gynecologic Oncologists of India.
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