Teriflunomide Loaded Spion Nanoparticles Induced Apoptosis in Mda-Mb-231 Breast Cancer Cells Publisher



Rabizadeh T¹ ; Varshochian R^{2, 3} ; Mahdieh A⁴ ; Rezaei M^{3, 4} ; Pazouki N¹ ; Zardkanlou M^{3, 4} ; Irani S¹ ; Dinarvand R^{3, 4} Show Affiliations
Source: Journal of Cluster Science Published:2023

Abstract

Introduction: Teriflunomide (TFN), an immunomodulatory drug, has demonstrated cytotoxic effects in recent studies. This potential can be combined with nano-drug delivery to achieve an improved anticancer formulation. Methods: In this study, super paramagnetic iron oxide nanoparticles (SPIONs) were coated with polydopamine (PD-SPIONs) and used to deliver TFN to the breast cancer cell lines, MDA-MB-231 and MCF-7. Nanoparticles were characterized in terms of size, polydispersity index, zeta potential, morphology. The cytotoxicity of PD-SPIONs, free-TFN and TFN loaded PD-SPIONs was investigated on the cell lines. MDA-MB-231 cells were examined for TP53 and BAX gene expression, cellular apoptosis, and cell cycle analysis. Methods: In this study, super paramagnetic iron oxide nanoparticles (SPIONs) were coated with polydopamine (PD-SPIONs) and used to deliver TFN to the breast cancer cell lines, MDA-MB-231 and MCF-7. Nanoparticles were characterized in terms of size, polydispersity index, zeta potential, morphology. The cytotoxicity of PD-SPIONs, free-TFN and TFN loaded PD-SPIONs was investigated on the cell lines. MDA-MB-231 cells were examined for TP53 and BAX gene expression, cellular apoptosis, and cell cycle analysis. Results: Nanoparticles with the size of 163 ± 8 nm and zeta potential of − 38 ± 4 mv were obtained. In comparison to cells treated with free-TFN, cells treated with TFN loaded PD-SPIONs showed significantly higher cytotoxicity. TFN loaded PD-SPIONs treated cells showed significant increase in TP53 (P < 0.001) and BAX (P < 0.01) gene expressions compared to free-TFN treated cells. MDA-MB-231 cells also showed an increased percentage of apoptosis and cell cycle arrest in the S and G2 phases. Conclusion: The designed TFN loaded nanoparticles showed promising potentials that can be considered in future breast cancer therapy research. © 2022, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.