Apoptosis Induction and Proliferation Inhibition by Silibinin Encapsulated in Nanoparticles in Mia Paca-2 Cancer Cells and Deregulation of Some Mirnas Publisher



Tehrani FK¹ ; Ranji N¹ ; Kouhkan F² ; Hosseinzadeh S³ Show Affiliations
Source: Iranian Journal of Basic Medical Sciences Published:2020

Abstract

Objective(s): Silibinin, as an herbal compound, has anti-cancer activity. Because of low solubility of silibinin in water and body fluids, it was encapsulated in polymersome nanoparticles and its effects were evaluated on pancreatic cancer cells and cancer stem cells. Materials and Methods: MIA PaCa-2 pancreatic cancer cells were treated with different doses of silibinin encapsulated in polymersome nanoparticles (SPNs). Stemness of MIA PaCa-2 cells was evaluated by hanging drop technique and CD133, CD24, and CD44 staining. The effects of SPNs on cell cycle, apoptosis and the expression of several genes and miRNAs were investigated. Results: IC50of SPNs was determined to be 40 μg/ml after 24 hr. Our analysis showed that >98% of MIA PaCa-2 cells expressed three stem cell markers. FACS analysis showed a decrease in these markers in SPNs-treated cells. PI/Annexin V staining revealed that 40 μg/ml and 50 μg/ml of SPNs increased apoptosis up to ∼40% and >80% of treated cells, respectively. Upregulation of miR-34a, miR-126, and miR-let7b and downregulation of miR-155, miR-222 and miR-21 was observed in SPNs-treated cells. In addition, downregulation of some genes involved in proliferation or migration such as AKT3, MASPINE, and SERPINEA12, and upregulation of apoptotic genes were observed in treated cells. Conclusion: Our results suggested that SPNs induced apoptosis and inhibited migration and proliferation in pancreatic cells and cancer stem cells through suppression of some onco-miRs and induction of some tumor suppressive miRs, as well as their targets. © 2020 Mashhad University of Medical Sciences. All rights reserved.