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Exosome Loaded Hydroxyapatite (Ha) Scaffold Promotes Bone Regeneration in Calvarial Defect: An in Vivo Study Publisher Pubmed



Youseflee P1 ; Ranjbar FE2 ; Bahraminasab M3 ; Ghanbari A4 ; Faradonbeh DR5 ; Arab S3 ; Alizadeh A3 ; Nooshabadi VT3
Authors
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Authors Affiliations
  1. 1. Medical Student, Student Research Committee, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran
  2. 2. Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
  3. 3. Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran
  4. 4. Research Center of Physiology, Semnan University of Medical Sciences, Semnan, Iran
  5. 5. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Cell and Tissue Banking Published:2023


Abstract

In this study, hydroxyapatite (HA) scaffolds were synthesized and characterized, following the osteogenic and angiogenic effects of HA scaffolds with or without endometrial mesenchymal stem stromal cells (hEnSCs) derived Exosomes were investigated in rat animal model with calvaria defect. The X-ray diffraction (XRD) analysis of HA powder formation was confirmed with Joint Corporation of Powder Diffraction Standards (JCPDS) files numbers of 34-0010 and 24-0033A and Ball mill, and sintering manufactured Nano-size particles. Obtained results containing FE-SEM images presented that the surface of scaffolds has a rough and porous structure, which makes them ideal and appropriate for tissue engineering. Additionally, the XRD showed that these scaffolds exhibited a crystallized structure without undergoing phase transformation; meanwhile, manufactured scaffolds consistently release exosomes; moreover, in vivo findings containing hematoxylin–eosin staining, immunohistochemistry, Masson's trichrome staining, and histomorphometric analysis confirmed that our implant has an osteogenic and angiogenic characteristic. So prepared scaffolds containing exosomes can be proposed as a promising substitute in tissue engineering. © 2022, The Author(s), under exclusive licence to Springer Nature B.V.
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