Expression Analysis of Long Non-Coding Atb and Its Putative Target in Breast Cancer Publisher Pubmed



Nikpayam E¹ ; Soudyab M¹ ; Tasharrofi B¹ ; Sarrafzadeh S¹ ; Iranpour M¹ ; Geranpayeh L² ; Mirfakhraie R¹ ; Gharesouran J¹ ; Ghafourifard S¹ Show Affiliations
Source: Breast Disease Published:2017

Abstract

BACKGROUND: A long noncoding RNA (lncRNA) activated by transforming growth factor (TGF)-β (lncRNA-ATB) has been recently shown to promote the invasion-metastasis cascade in various types of cancers via upregulation of some targets including ZEB1. OBJECTIVES: The aim of the present study was to elucidate the expression of lncRNA-ATB and ZEB in breast cancer patients. METHODS: The expression of these genes was evaluated by real-time reverse transcription polymerase chain reaction in tumor samples form 50 newly diagnosed breast cancer patients as well as their corresponding adjacent non-cancerous tissues (ANCTs). Patients were divided into subsequent groups according to the median lncRNA-ATB expression. RESULTS: LncRNA-ATB has been shown to be downregulated in about two third of tumor samples compared with their ANCTs. A significant association has been found between ZEB1 expression and Ki-67 status. In addition, we demonstrated a correlation between expression of lncRNA-ATB and ZEB1 in tumor samples and not in ANCTs. CONCLUSION: Collectively, out data show downregulation of lncRNA-ATB in a significant number of breast tumor tissues compared with ANCTs and imply that lncRNA-ATB might have distinct roles in the pathogenesis of different cancers or even different subtypes of a certain cancer which should be evaluated in future studies. © 2017 - IOS Press and the authors.