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Effect of Duration of Root Canal Infection on the Ability of Dentin-Pulp Complex Regeneration of Immature Permanent Teeth: An Animal Study Publisher Pubmed



Iranmanesh P1 ; Torabinejad M2 ; Saatchi M1 ; Toghraie D3 ; Razavi SM4 ; Khademi A1
Authors
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Authors Affiliations
  1. 1. Department of Endodontics, Dental Research Center, School of Dentistry, Dental Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. School of Dentistry, Loma Linda University, Loma Linda, California, United States
  3. 3. Department of Mechanical Engineering, Khomeinishahr Branch, Islamic Azad University, Khomeinishahr, Iran
  4. 4. Department of Oral and Maxillofacial Pathology and Dental Implants Research Center, Dental Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Endodontics Published:2022


Abstract

Introduction: Persistent infection is always considered the most important reason for the failure of dentin-pulp complex regeneration. The present study aimed to evaluate the effect of the duration of root canal infection (from 1–12 weeks) on the ability of dentin-pulp complex regeneration. Methods: In this animal study, 64 roots of immature premolar teeth of 4 dogs were randomly divided into the following groups: the positive control group, 8 root canals treated with the regenerative endodontic procedure (REP); the negative control group, 12 infected root canals; the intervention groups, 36 root canals infected with supragingival plaque (1, 3, 6, and 12 weeks) and treated with REP; and an additional positive control group, 8 normal roots. After 3 months, the teeth were investigated by radiographic images and immunohistochemical staining (CD31, CD34, and S100 markers). In addition, DSPP gene expression was assessed using a real-time polymerase chain reaction technique. Results: Based on radiologic evaluation among the intervention groups, the highest root canal development (length and width) occurred in the intervention group of 1 week, and the lowest radiologic results were in the intervention groups of 6 and 12 weeks (1-way analysis of variance, P-value < .05). There was a significant difference between the groups in terms of CD31, CD34, S100, and DSPP expression percentage (1-way analysis of variance, P-value < .05); the highest and lowest expression percentages belonged to the 1- and 12-week groups, respectively, among the intervention groups. Conclusions: This study demonstrated that long root canal infection decreased the ability of the body to regenerate the dentin-pulp complex. © 2022 American Association of Endodontists
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