Associations of a Tlr4 Single-Nucleotide Polymorphism With H.Pylori Associated Gastric Diseases in Iranian Patients Publisher Pubmed



Bagheri N¹ ; Azadegandehkordi F² ; Sanei H³ ; Taghikhani A⁴ ; Rahimian G⁴ ; Salimzadeh L² ; Hashemzadehchaleshtori M² ; Rafieiankopaei M⁵ ; Shirzad M⁵ ; Shirzad H² Show Affiliations
Source: Clinics and Research in Hepatology and Gastroenterology Published:2014

Abstract

Objective: Helicobacter pylori (H.pylori) is associated with gastric ulcer and gastric adenocarcinoma. Polymorphisms in the host genes coding for toll-like receptors (TLRs) may influence the innate and adaptive immune response to the infection, affecting the susceptibility to H.pylori or the disease outcomes. But the details and association to different polymorphisms and different clinical expressions in patients infected with H.pylori (different clinical expression of H.pylori infection) remain unclear. Methods: A case-control study consisting of 195 patients with H.pylori infection and 241 H.pylori uninfection was conducted. Genomic DNA was extracted and genotypes of TLR4Asp299Gly polymorphism were assessed through polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Presence of cagA was evaluated using PCR. Results: TLR4 (Asp299Gly) G and DG alleles frequency in H.pylori infected population was signifi;cantly higher in the chronic gastritis group than in the chronic active gastritis group (P=0.021; OR, 2.409; 95% CI, 1.124-5.162). Grade mononuclear (MN) infiltration in H.pylori infected patients with DG genotype of TLR-4 Asp299Gly increased significantly. CagA positivity was more frequently associated with chronic active gastritis (P=0.017, OR=2.26, 95% CI=1.144-4.462) and grade polymorphonucler (PMN) infiltration. Conclusion: TLR-4 Asp299Gly G allele substitution may be modified pattern of immune response in the gastric mucosa of H.pylori infected patients and may be H.pylori infected patients with gastritis have increased risk for the development of chronic gastritis. CagA positivity may be a risk factor for development of gastritis. © 2013 Elsevier Masson SAS.