Comparison of Helicobacter Pylori and Escherichia Coli in Induction of Tnf-Α Mrna From Human Peripheral Blood Mononuclear Cells Publisher Pubmed



Ahmadzadeh E¹ ; Zarkeshesfahani H¹ ; Roghanian R¹ ; Akbar FN²
Source: Indian Journal of Medical Microbiology Published:2010

Abstract

Purpose: To investigate the difference between the abilities of Helicobacter pylori and Escherichia coli to induce expression of TNF-αin human peripheral blood mononuclear cells (PBMC). Materials and Methods: H pylori was isolated from gastric biopsy specimens. The mononuclear cells were isolated from human blood, cultured, and treated with either intact or sonicated E coli or H pylori, and mRNA expression for TNF-αwas detected using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Results: TNF-αmRNA expression levels were significantly higher in PBMCs stimulated with E coli compared to those stimulated with H pylori at the same number and identical conditions (P <.001). The results also suggest that sonicated bacteria were significantly (P <.001) less stimulatory for PBMCs than intact bacteria for both E coli and H pylori. Conclusions: The ability of different H pylori strains isolated from biopsy samples to stimulate TNF-α from PBMCs was significantly lower than that of E coli. Sonicated bacteria, as compared to intact bacteria, was a very poor inducer of TNF-αmRNA expression, suggesting that the conformation of lipopolysaccharides (LPS) on the outer leaflet of the outer membrane is not totally conserved in sonicated bacteria.