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Cell Attachment and Proliferation of Human Adipose-Derived Stem Cells on Plga/Chitosan Electrospun Nano-Biocomposite



Razavi S1 ; Karbasi S2 ; Morshed M3 ; Esfahani HZ4 ; Golozar M5 ; Vaezifar S1, 5
Authors
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Authors Affiliations
  1. 1. Department of Anatomical Sciences and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, P.O.Box: 81744-176, Isfahan, Iran
  2. 2. Department of Medical Physics and Biomedical Engineering, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Textile Engineering, Isfahan University of Technology, Isfahan, Iran
  4. 4. Department of Biology, Faculty of Sciences, University of Isfahan, Isfahan, Iran
  5. 5. Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran

Source: Cell Journal Published:2015

Abstract

Objective: In this study, nano-biocomposite composed of poly (lactide-co-glycolide) (PLGA) and chitosan (CS) were electrospun through a single nozzle by dispersing the CS nano-powders in PLGA solution. The cellular behavior of human adipose derived stem cells (h-ADSCs) on random and aligned scaffolds was then evaluated. Materials and Methods: In this experimental study, the PLGA/CS scaffolds were prepared at the different ratios of 90/10, 80/20, and 70/30 (w/w) %. Morphology, cell adhesion and proliferation rate of h-ADSCs on the scaffolds were assessed using scanning electron microscope (SEM), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay and trypan blue staining respectively. Results: H-ADSCs seeded on the matrices indicated that the PLGA/CS composite matrix with aligned nanofibres and higher content of CS nano-powders gave significantly better performance than others in terms of cell adhesion and proliferation rate (P<0.05). Conclusion: We found that CS enhanced cell adhesion and proliferation rate, and aligned nanofibers guided cell growth along the longitudinal axis of the nanofibers, which would provide a beneficial approach for tissue engineering.
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