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Efficient Co-Cultivation of Human Fibroblast Cells (Hfcs) and Adipose-Derived Stem Cells (Adss) on Gelatin/Plcl Nanofiber Publisher Pubmed



Ranjbarmohammadi M1 ; Mousavi E2 ; Hashemi MM3 ; Abbasian M3 ; Asadi J4 ; Esmaili E5 ; Fesharaki M5 ; Asadi P6 ; Arabbafrani Z3, 7, 8
Authors
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Authors Affiliations
  1. 1. Textile Engineering Group, Faculty of Engineering, University of Bonab, Bonab, Iran
  2. 2. Department of Medical Microbiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran
  3. 3. Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran
  4. 4. Health Management and Social Development Research Center, Golestan University of Medical Sciences, Gorgan, Iran
  5. 5. Department of Cell Sciences Research Center Medical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  6. 6. Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran
  7. 7. Department of Biochemistry and Biophysics, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran
  8. 8. Health Technology Research Center, Oxin Sabz Espadan Company, Esfahan University of Medical Sciences, Iran

Source: IET Nanobiotechnology Published:2020


Abstract

In this study, we investigated whether the nanofibers produced by natural-synthetic polymers can probably promote the proliferation of co-cultured adipose-derived stem cells/human fibroblast cells (ADSs/HFCs) and synthesis of collagen. Nanofiber was fabricated by blending gelatin and poly (L-lactide co-ϵ-caprolactone) (PLCL) polymer nanofiber (Gel/PLCL). Cell morphology and the interaction between cells and Gel/PLCL nanofiber were evaluated by FESEM and fluorescent microscopy. MTS assay and quantitative real-time polymerase chain reaction were applied to assess the proliferation of co-cultured ADSs/ HFCs and the collagen type I and III synthesis, respectively. The concentrations of two cytokines including fibroblast growth factor-basic and transforming growth factor-β1 were also measured in culture medium of co-cultured ADSs/HDCs using enzyme-linked immunosorbent assay assay. Actually, nanofibers exhibited proper structural properties in terms of stability in cell proliferation and toxicity analysis processes. Gel/PLCL nanofiber promoted the growth and the adhesion of HFCs. Our results showed in contact co-culture of ADSs/HFCs on the Gel/PLCL nanofiber increased cellular adhesion and proliferation synergistically compared to non-coated plate. Also, synthesis of collagen and cytokines secretion of co-cultured ADSs/HFCs on Gel/PLCL scaffolds is significantly higher than non-coated plates. To conclude, the results suggest that Gel/PLCL nanofiber can imitate physiological characteristics in vivo and enhance the efficacy of co-cultured ADSs/HFCs in wound healing process. © The Institution of Engineering and Technology 2019.
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