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Echinococcus Granulosus Sheep Strain (G1) As the Predominant Genotype in Definitive Host (Dogs) Isolates in Northeastern Iran Publisher Pubmed



Hejazi SH1 ; Mirbadie SR2 ; Jafari R3 ; Rezaiemanesh MR4, 5 ; Azizi O4, 5 ; Badmasti F6 ; Kalani H7 ; Cheraghipour K8 ; Heydarian P9 ; Hashemi N10 ; Izadi S11 ; Jabalameli Z12 ; Mohaghegh MA4, 5, 13
Authors
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Authors Affiliations
  1. 1. Department of Medical Parasitology and Mycology, School of Medicine, Skin Diseases and Leishmaniasis Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
  3. 3. Department of Medical Parasitology and Mycology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran
  4. 4. Department of Laboratory Sciences, School of Paramedical Sciences, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
  5. 5. Health Sciences Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
  6. 6. Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran
  7. 7. Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran
  8. 8. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
  9. 9. Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
  10. 10. School of Medicine, Bam University of Medical Sciences, Bam, Iran
  11. 11. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  12. 12. Vice Chancellery of Education and Research, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran
  13. 13. Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Veterinary Parasitology: Regional Studies and Reports Published:2024


Abstract

Echinococcus granulosus sensu lato (E. granulosus s.l.) is a zoonotic parasite, causing cystic echinococcosis in humans. In the present study, prevalence and genotypes of E. granulosus s.l. was assessed in stools collected from 244 dogs including 138 stray and 106 domestic animals using high resolution melting curve (HRM) method. Initially, to detect taeniid eggs in feces, all samples were examined using the formalin-ether techniques. Genomic DNA was extracted from the positive samples and E. granulosus s.l. was differentiated from other Taeniidae parasites using SSU-rDNA gene and E. granulosus s.l. was analyzed for genotyping using HRM based on the cox1 gene. In total, 12.7% (31/244) of the samples were positive for Taeniidae eggs. In addition, among the positive samples, 77.4% (24/31) were positive for E. granulosus s.l. In details, 11.3% (12/106) of the domestic dogs and 8.7% (12/138) of the stray dogs were positive for E. granulosus s.l. The results of HRM analysis showed that all E. granulosus s.l. isolates were G1 strain. Findings of the present study indicated a considerable prevalence of E. granulosus G1 among dogs in the northeast of Iran and imply a serious risk of transmitting to humans and livestock. © 2023
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