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A New Primer Pair in Its1 Region for Molecular Studies on Echinococcus Granulosus



Rahimi HR1 ; Kia EB1 ; Mirhendi SH1 ; Talebi A2 ; Harandi MF3 ; Jalalizand N1 ; Rokni MB1
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Authors Affiliations
  1. 1. Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Al-Zahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Dept. of Medical Parasitology and Mycology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Source: Iranian Journal of Public Health Published:2007

Abstract

Background: Echinocuccus granulosus, the causative agent of cystic echinococcosis has long been recognized as having a high degree of genetic divergence. The strains characterization seems to be essential for the establishment of a preventive and control strategy in every endemic area. Using DNA based methods for strain/genotype characterizations of E. granulosus have some difficulties, especially access to an efficient and pure concentration of DNA and proper primes. Methods: Using grinder method, a pure and high concentration DNA was extracted from 10 human, hydatid cysts collected from Isfahan (central Iran) hospitals, and processed for PCR reaction. Results: Using DNASIS, the primers were designed in internal transcribed spacer I (ITSI) region, following analysis of 30 E. granulosus nucleotide sequences, extracted from gene bank. Conclusion: This new and specific E. granulosus primer, which amplified DNA thoroughly can be applied for molecular studies on echinococcosis.
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